Pomegranate-Derived Polyphenols Reduce Reactive Oxygen Species Production via SIRT3-Mediated SOD2 Activation

Chong Zhao, Takenori Sakaguchi, Kosuke Fujita, Hideyuki Ito, Norihisa Nishida, Akifumi Nagatomo, Yukimasa Tanaka-Azuma, Yoshinori Katakura

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Pomegranate-derived polyphenols are expected to prevent life-style related diseases. In this study, we evaluated the ability of 8 pomegranate-derived polyphenols, along with other polyphenols, to augment SIRT3, a mammalian SIR2 homolog localized in mitochondria. We established a system for screening foods/food ingredients that augment the SIRT3 promoter in Caco-2 cells and identified 3 SIRT3-augmenting pomegranate-derived polyphenols (eucalbanin B, pomegraniin A, and eucarpanin T1). Among them, pomegraniin A activated superoxide dismutase 2 (SOD2) through SIRT3-mediated deacetylation, thereby reducing intracellular reactive oxygen species. The other SIRT3-augmenting polyphenols tested also activated SOD2, suggesting antioxidant activity. Our findings clarify the underlying mechanisms involved in the antioxidant activity of pomegraniin A.

Original languageEnglish
Article number2927131
JournalOxidative medicine and cellular longevity
Volume2016
DOIs
Publication statusPublished - Jan 1 2016

Fingerprint

Punicaceae
Polyphenols
Reactive Oxygen Species
Chemical activation
Antioxidants
Food
Mitochondria
Aptitude
Caco-2 Cells
Life Style
Screening
superoxide dismutase 2

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Ageing
  • Cell Biology

Cite this

Pomegranate-Derived Polyphenols Reduce Reactive Oxygen Species Production via SIRT3-Mediated SOD2 Activation. / Zhao, Chong; Sakaguchi, Takenori; Fujita, Kosuke; Ito, Hideyuki; Nishida, Norihisa; Nagatomo, Akifumi; Tanaka-Azuma, Yukimasa; Katakura, Yoshinori.

In: Oxidative medicine and cellular longevity, Vol. 2016, 2927131, 01.01.2016.

Research output: Contribution to journalArticle

Zhao, Chong ; Sakaguchi, Takenori ; Fujita, Kosuke ; Ito, Hideyuki ; Nishida, Norihisa ; Nagatomo, Akifumi ; Tanaka-Azuma, Yukimasa ; Katakura, Yoshinori. / Pomegranate-Derived Polyphenols Reduce Reactive Oxygen Species Production via SIRT3-Mediated SOD2 Activation. In: Oxidative medicine and cellular longevity. 2016 ; Vol. 2016.
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AU - Nishida, Norihisa

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AU - Tanaka-Azuma, Yukimasa

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