Porcine dental epithelial cells differentiated in a cell sheet constructed by magnetic nanotechnology

Wataru Koto, Yoshinori Shinohara, Kazuyuki Kitamura, Takanori Wachi, Seicho Makihira, Kiyoshi Koyano

Research output: Contribution to journalArticle

Abstract

Magnetic nanoparticles (MNPs) are widely used in medical examinations, treatments, and basic research, including magnetic resonance imaging, drug delivery systems, and tissue engineering. In this study, MNPs with magnetic force were applied to tissue engineering for dental enamel regeneration. The internalization of MNPs into the odontogenic cells was observed by transmission electron microscopy. A combined cell sheet consisting of dental epithelial cells (DECs) and dental mesenchymal cells (DMCs) (CC sheet) was constructed using magnetic force-based tissue engineering technology. The result of the iron staining indicated that MNPs were distributed ubiquitously over the CC sheet. mRNA expression of enamel differentiation and basement membrane markers was examined in the CC sheet. Immunostaining showed Collagen IV expression at the border region between DEC and DMC layers in the CC sheet. These results revealed that epithelial-mesenchymal interactions between DEC and DMC layers were caused by bringing DECs close to DMCs mechanically by magnetic force. Our study suggests that the microenvironment in the CC sheet might be similar to that during the developmental stage of a tooth bud. In conclusion, a CC sheet employing MNPs could be developed as a novel and unique graft for artificially regenerating dental enamel.

Original languageEnglish
Article number322
JournalNanomaterials
Volume7
Issue number10
DOIs
Publication statusPublished - Oct 13 2017

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Nanotechnology
Enamels
Nanoparticles
Tissue engineering
Engineering technology
Magnetic resonance
Systems engineering
Collagen
Grafts
Iron
Epithelial Cells
Transmission electron microscopy
Imaging techniques
Messenger RNA

All Science Journal Classification (ASJC) codes

  • Materials Science(all)
  • Chemical Engineering(all)

Cite this

Porcine dental epithelial cells differentiated in a cell sheet constructed by magnetic nanotechnology. / Koto, Wataru; Shinohara, Yoshinori; Kitamura, Kazuyuki; Wachi, Takanori; Makihira, Seicho; Koyano, Kiyoshi.

In: Nanomaterials, Vol. 7, No. 10, 322, 13.10.2017.

Research output: Contribution to journalArticle

Koto, Wataru ; Shinohara, Yoshinori ; Kitamura, Kazuyuki ; Wachi, Takanori ; Makihira, Seicho ; Koyano, Kiyoshi. / Porcine dental epithelial cells differentiated in a cell sheet constructed by magnetic nanotechnology. In: Nanomaterials. 2017 ; Vol. 7, No. 10.
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