Porcine follistatin gene structure supports two forms of mature follistatin produced by alternative splicing

Shunichi Shimasaki, Makoto Koga, Fred Esch, Maluz Mercado, Karen Cooksey, Ann Koba, Nicholas Ling

Research output: Contribution to journalArticle

90 Citations (Scopus)

Abstract

Follistatin (FS), a novel gonadal protein which inhibits specifically the secretion of pituitary follicle stimulating hormone(FSH), has recently been isolated from porcine follicular fluid, cDNA cloning of the porcine ovarian FS precursor revealed two populations of cDNAs which differed at the 3′-region of the open reading frames; one population encodes a precursor of 317 amino acids while the other encodes another precursor having the same 317 amino acids, but with an additional 27 amino acids at the carboxy-terminal. Herein, we report the cloning of the porcine FS gene whose DNA structure reveals that the two populations of mRNA are generated by alternative splicing. In addition, restriction endonuclease mapping and DNA sequencing show that the FS gene is approximately 6 Kb long and consists of six exons separated by five introns. The first exon encodes the putative signal sequence, followed by four exons which encode the four domains of FS, three of which are highly homologous to each other. The last exon encodes the extra 27-amino acid carboxy-terminal domain of the 344-residued precursor.

Original languageEnglish
Pages (from-to)717-723
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume152
Issue number2
DOIs
Publication statusPublished - Apr 29 1988

Fingerprint

Follistatin
Alternative Splicing
Swine
Genes
Exons
Amino Acids
Cloning
Organism Cloning
Complementary DNA
Population
Follicular Fluid
Restriction Mapping
Pituitary Hormones
DNA
DNA Restriction Enzymes
Follicle Stimulating Hormone
Protein Sorting Signals
DNA Sequence Analysis
Introns
Open Reading Frames

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Porcine follistatin gene structure supports two forms of mature follistatin produced by alternative splicing. / Shimasaki, Shunichi; Koga, Makoto; Esch, Fred; Mercado, Maluz; Cooksey, Karen; Koba, Ann; Ling, Nicholas.

In: Biochemical and Biophysical Research Communications, Vol. 152, No. 2, 29.04.1988, p. 717-723.

Research output: Contribution to journalArticle

Shimasaki, Shunichi ; Koga, Makoto ; Esch, Fred ; Mercado, Maluz ; Cooksey, Karen ; Koba, Ann ; Ling, Nicholas. / Porcine follistatin gene structure supports two forms of mature follistatin produced by alternative splicing. In: Biochemical and Biophysical Research Communications. 1988 ; Vol. 152, No. 2. pp. 717-723.
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