Porphyromonas gingivalis, a cause of preterm birth in mice, induces an inflammatory response in human amnion mesenchymal cells but not epithelial cells

Haruhisa Konishi, Satoshi Urabe, Yuko Teraoka, Yoshito Morishita, Iemasa Koh, Jun Sugimoto, Shinnichi Sakamoto, Hiroshi Miyoshi, Mutsumi Miyauchi, Takashi Takata, Shunichi Kajioka, Yoshiki Kudo

Research output: Contribution to journalArticle

Abstract

Introduction: Inflammation and infection, including dental infectious diseases, are factors that can induce preterm birth. We previously reported that mice with dental Porphyromonas gingivalis infection could be used as a model of preterm birth. In this model, cyclooxygenase (COX)-2 and interleukin (IL)-1β levels are increased, and P. gingivalis colonies are observed in the fetal membrane. However, the mechanism underlying fetal membrane inflammation remains unknown. Therefore, we investigated the immune responses of human amnion to P. gingivalis in vitro. Methods: Epithelial and mesenchymal cells were isolated from human amnion using trypsin and collagenase, and primary cell cultures were obtained. Confluent cells were stimulated with P. gingivalis lipopolysaccharide (P.g-LPS) or P. gingivalis. mRNA expressions of IL-1β, IL-8, IL-6 and COX-2, protein expressions of nuclear factor (NF)-κB pathway components and culture medium levels of prostaglandin E2 were evaluated. Results: Following stimulation with 1 μg/mL P.g-LPS, the mRNA expression levels of IL-1β, IL-8, IL-6 and COX-2 in mesenchymal cells were increased 5.9-, 3.3-, 4.2- and 3.1-fold, respectively. Similarly, the expression levels of IL-1β, IL-8, IL-6 and COX-2 in mesenchymal cells were increased by 7.6-, 8.2-, 13.4- and 9.3-fold, respectively, after coculture with P. gingivalis. Additionally, stimulation with P.g-LPS or P. gingivalis resulted in the activation of NF-κB signaling and increased production of IL-1β and prostaglandin E2. In contrast, no significant changes were observed in epithelial cells. Discussion: Our findings suggest that mesenchymal cells might mediate the inflammatory responses to P. gingivalis and P.g-LPS, thereby producing inflammation that contributes to the induction of preterm birth.

Original languageEnglish
Pages (from-to)21-26
Number of pages6
JournalPlacenta
Volume99
DOIs
Publication statusPublished - Sep 15 2020

All Science Journal Classification (ASJC) codes

  • Reproductive Medicine
  • Obstetrics and Gynaecology
  • Developmental Biology

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    Konishi, H., Urabe, S., Teraoka, Y., Morishita, Y., Koh, I., Sugimoto, J., Sakamoto, S., Miyoshi, H., Miyauchi, M., Takata, T., Kajioka, S., & Kudo, Y. (2020). Porphyromonas gingivalis, a cause of preterm birth in mice, induces an inflammatory response in human amnion mesenchymal cells but not epithelial cells. Placenta, 99, 21-26. https://doi.org/10.1016/j.placenta.2020.07.016