Porphyromonas pasteri sp. Nov., isolated from human saliva

Mitsuo Sakamoto, Dan Li, Yukie Shibata, Toru Takeshita, Yoshihisa Yamashita, Moriya Ohkuma

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

A bacterial strain, designated KUFDS01T, isolated from human saliva was characterized using a polyphasic taxonomic approach that included analysis of physiological and biochemical features, cellular fatty acid profiles and phylogenetic position based on 16S rRNA gene sequence analysis. Cells of the strain were obligately anaerobic, non-pigmented, non-spore-forming, non-motile, Gram-stain-negative rods. Growth of the strain was inhibited on medium containing 20% bile. The 16S rRNA gene sequence analysis showed that the strain was a member of the genus Porphyromonas. Strain KUFDS01T was closely related to Porphyromonas catoniae JCM 13863T (96.6% sequence similarity). An hsp60 gene sequence analysis indicated that strain KUFDS01T was different from P. catoniae JCM 13863T, with a sequence similarity value of 87.8%. The major cellular fatty acids of strain KUFDS01T were C16:0, iso-C15:0, anteiso-C15:0,C18: 2ω6, 9c and C18: 1ω9c. The DNA G + C content of strain KUFDS01T was 57.7 + 0.66 mol%. On the basis of these data, strain KUFDS01T represents a novel species of the genus Porphyromonas, for which the name Porphyromonas pasteri sp. nov. is proposed. The type strain of P. pasteri is KUFDS01T (=JCM 30531T=CCUG 66735T).

Original languageEnglish
Pages (from-to)2511-2515
Number of pages5
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume65
Issue number8
DOIs
Publication statusPublished - Aug 1 2015

Fingerprint

Porphyromonas
saliva
Saliva
Sequence Analysis
rRNA Genes
gene
Fatty Acids
fatty acid
Base Composition
Bile
sequence analysis
Names
nucleotide sequences
phylogenetics
DNA
analysis
ribosomal RNA
Growth
Genes
bile

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

Cite this

Porphyromonas pasteri sp. Nov., isolated from human saliva. / Sakamoto, Mitsuo; Li, Dan; Shibata, Yukie; Takeshita, Toru; Yamashita, Yoshihisa; Ohkuma, Moriya.

In: International Journal of Systematic and Evolutionary Microbiology, Vol. 65, No. 8, 01.08.2015, p. 2511-2515.

Research output: Contribution to journalArticle

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