Abstract
A bacterial strain, designated KUFDS01T, isolated from human saliva was characterized using a polyphasic taxonomic approach that included analysis of physiological and biochemical features, cellular fatty acid profiles and phylogenetic position based on 16S rRNA gene sequence analysis. Cells of the strain were obligately anaerobic, non-pigmented, non-spore-forming, non-motile, Gram-stain-negative rods. Growth of the strain was inhibited on medium containing 20% bile. The 16S rRNA gene sequence analysis showed that the strain was a member of the genus Porphyromonas. Strain KUFDS01T was closely related to Porphyromonas catoniae JCM 13863T (96.6% sequence similarity). An hsp60 gene sequence analysis indicated that strain KUFDS01T was different from P. catoniae JCM 13863T, with a sequence similarity value of 87.8%. The major cellular fatty acids of strain KUFDS01T were C16:0, iso-C15:0, anteiso-C15:0,C18: 2ω6, 9c and C18: 1ω9c. The DNA G + C content of strain KUFDS01T was 57.7 + 0.66 mol%. On the basis of these data, strain KUFDS01T represents a novel species of the genus Porphyromonas, for which the name Porphyromonas pasteri sp. nov. is proposed. The type strain of P. pasteri is KUFDS01T (=JCM 30531T=CCUG 66735T).
| Original language | English |
|---|---|
| Pages (from-to) | 2511-2515 |
| Number of pages | 5 |
| Journal | International Journal of Systematic and Evolutionary Microbiology |
| Volume | 65 |
| Issue number | 8 |
| DOIs | |
| Publication status | Published - Aug 1 2015 |
| Externally published | Yes |
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All Science Journal Classification (ASJC) codes
- Microbiology
- Ecology, Evolution, Behavior and Systematics
Cite this
Porphyromonas pasteri sp. Nov., isolated from human saliva. / Sakamoto, Mitsuo; Li, Dan; Shibata, Yukie; Takeshita, Toru; Yamashita, Yoshihisa; Ohkuma, Moriya.
In: International Journal of Systematic and Evolutionary Microbiology, Vol. 65, No. 8, 01.08.2015, p. 2511-2515.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Porphyromonas pasteri sp. Nov., isolated from human saliva
AU - Sakamoto, Mitsuo
AU - Li, Dan
AU - Shibata, Yukie
AU - Takeshita, Toru
AU - Yamashita, Yoshihisa
AU - Ohkuma, Moriya
PY - 2015/8/1
Y1 - 2015/8/1
N2 - A bacterial strain, designated KUFDS01T, isolated from human saliva was characterized using a polyphasic taxonomic approach that included analysis of physiological and biochemical features, cellular fatty acid profiles and phylogenetic position based on 16S rRNA gene sequence analysis. Cells of the strain were obligately anaerobic, non-pigmented, non-spore-forming, non-motile, Gram-stain-negative rods. Growth of the strain was inhibited on medium containing 20% bile. The 16S rRNA gene sequence analysis showed that the strain was a member of the genus Porphyromonas. Strain KUFDS01T was closely related to Porphyromonas catoniae JCM 13863T (96.6% sequence similarity). An hsp60 gene sequence analysis indicated that strain KUFDS01T was different from P. catoniae JCM 13863T, with a sequence similarity value of 87.8%. The major cellular fatty acids of strain KUFDS01T were C16:0, iso-C15:0, anteiso-C15:0,C18: 2ω6, 9c and C18: 1ω9c. The DNA G + C content of strain KUFDS01T was 57.7 + 0.66 mol%. On the basis of these data, strain KUFDS01T represents a novel species of the genus Porphyromonas, for which the name Porphyromonas pasteri sp. nov. is proposed. The type strain of P. pasteri is KUFDS01T (=JCM 30531T=CCUG 66735T).
AB - A bacterial strain, designated KUFDS01T, isolated from human saliva was characterized using a polyphasic taxonomic approach that included analysis of physiological and biochemical features, cellular fatty acid profiles and phylogenetic position based on 16S rRNA gene sequence analysis. Cells of the strain were obligately anaerobic, non-pigmented, non-spore-forming, non-motile, Gram-stain-negative rods. Growth of the strain was inhibited on medium containing 20% bile. The 16S rRNA gene sequence analysis showed that the strain was a member of the genus Porphyromonas. Strain KUFDS01T was closely related to Porphyromonas catoniae JCM 13863T (96.6% sequence similarity). An hsp60 gene sequence analysis indicated that strain KUFDS01T was different from P. catoniae JCM 13863T, with a sequence similarity value of 87.8%. The major cellular fatty acids of strain KUFDS01T were C16:0, iso-C15:0, anteiso-C15:0,C18: 2ω6, 9c and C18: 1ω9c. The DNA G + C content of strain KUFDS01T was 57.7 + 0.66 mol%. On the basis of these data, strain KUFDS01T represents a novel species of the genus Porphyromonas, for which the name Porphyromonas pasteri sp. nov. is proposed. The type strain of P. pasteri is KUFDS01T (=JCM 30531T=CCUG 66735T).
UR - http://www.scopus.com/inward/record.url?scp=84941079455&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84941079455&partnerID=8YFLogxK
U2 - 10.1099/ijs.0.000294
DO - 10.1099/ijs.0.000294
M3 - Article
C2 - 25933621
AN - SCOPUS:84941079455
VL - 65
SP - 2511
EP - 2515
JO - International Journal of Systematic and Evolutionary Microbiology
JF - International Journal of Systematic and Evolutionary Microbiology
SN - 1466-5026
IS - 8
ER -