Porphyromonas pasteri sp. Nov., isolated from human saliva

A bacterial strain, designated KUFDS01^T, isolated from human saliva was characterized using a polyphasic taxonomic approach that included analysis of physiological and biochemical features, cellular fatty acid profiles and phylogenetic position based on 16S rRNA gene sequence analysis. Cells of the strain were obligately anaerobic, non-pigmented, non-spore-forming, non-motile, Gram-stain-negative rods. Growth of the strain was inhibited on medium containing 20% bile. The 16S rRNA gene sequence analysis showed that the strain was a member of the genus Porphyromonas. Strain KUFDS01^T was closely related to Porphyromonas catoniae JCM 13863^T (96.6% sequence similarity). An hsp60 gene sequence analysis indicated that strain KUFDS01T was different from P. catoniae JCM 13863^T, with a sequence similarity value of 87.8%. The major cellular fatty acids of strain KUFDS01^T were C₁₆:₀, iso-C₁₅:₀, anteiso-C₁₅:₀,C₁₈: 2^ω6, 9c and C₁₈: 1^ω9c. The DNA G + C content of strain KUFDS01^T was 57.7 + 0.66 mol%. On the basis of these data, strain KUFDS01^T represents a novel species of the genus Porphyromonas, for which the name Porphyromonas pasteri sp. nov. is proposed. The type strain of P. pasteri is KUFDS01^T (=JCM 30531^T=CCUG 66735^T).