Positional cloning of silkworm white egg 2 (w-2) locus shows functional conservation and diversification of ABC transporters for pigmentation in insects

Ken ichiro Tatematsu, Kimiko Yamamoto, Keiro Uchino, Junko Narukawa, Tetsuya Iizuka, Yutaka Banno, Susumu Katsuma, Toru Shimada, Toshiki Tamura, Hideki Sezutsu, Takaaki Daimon

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

The white, scarlet and brown genes of Drosophila melanogaster encode three half-type ATP-binding cassette (ABC) transporters. In Drosophila, precursors of ommochromes and pteridines are transported by White/Scarlet and White/Brown heterodimers, respectively. The white egg 2 (w-2) mutant of the silkworm, Bombyx mori, has white eggs and eyes because of lack of ommochrome granules in the serosa and eyes. Here, we report that the silkworm w-2 locus encodes an ortholog of Drosophila scarlet. Our results indicate that Bombyx Scarlet forms a heterodimer with Bombyx White to transport ommochrome precursors, suggesting that formation of a White/Scarlet heterodimer and its involvement in the transport of ommochrome precursors are evolutionarily ancient and widely conserved traits in insects. Contrary to dipteran insects, white and scarlet were juxtaposed in a head-to-tail orientation in the silkworm genome, suggesting that the origin of white and scarlet was a tandem duplication of their ancestral transporter gene. In Bombyx, White is also essential for the transport of uric acid in larval epidermis. However, our results suggest that a Bombyx White/Scarlet heterodimer is not involved in this process. Our results emphasize the functional conservation and diversification of half-type ABC transporter families in insects, which may contribute to their extremely diverse color patterns.

Original languageEnglish
Pages (from-to)331-342
Number of pages12
JournalGenes to Cells
Volume16
Issue number4
DOIs
Publication statusPublished - Apr 1 2011

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Egg White
ATP-Binding Cassette Transporters
Bombyx
Pigmentation
Insects
Organism Cloning
Drosophila
Pteridines
Serous Membrane
Uric Acid
Drosophila melanogaster
Epidermis
Genes
Color
Genome
ommochrome

All Science Journal Classification (ASJC) codes

  • Genetics
  • Cell Biology

Cite this

Positional cloning of silkworm white egg 2 (w-2) locus shows functional conservation and diversification of ABC transporters for pigmentation in insects. / Tatematsu, Ken ichiro; Yamamoto, Kimiko; Uchino, Keiro; Narukawa, Junko; Iizuka, Tetsuya; Banno, Yutaka; Katsuma, Susumu; Shimada, Toru; Tamura, Toshiki; Sezutsu, Hideki; Daimon, Takaaki.

In: Genes to Cells, Vol. 16, No. 4, 01.04.2011, p. 331-342.

Research output: Contribution to journalArticle

Tatematsu, KI, Yamamoto, K, Uchino, K, Narukawa, J, Iizuka, T, Banno, Y, Katsuma, S, Shimada, T, Tamura, T, Sezutsu, H & Daimon, T 2011, 'Positional cloning of silkworm white egg 2 (w-2) locus shows functional conservation and diversification of ABC transporters for pigmentation in insects', Genes to Cells, vol. 16, no. 4, pp. 331-342. https://doi.org/10.1111/j.1365-2443.2011.01490.x
Tatematsu, Ken ichiro ; Yamamoto, Kimiko ; Uchino, Keiro ; Narukawa, Junko ; Iizuka, Tetsuya ; Banno, Yutaka ; Katsuma, Susumu ; Shimada, Toru ; Tamura, Toshiki ; Sezutsu, Hideki ; Daimon, Takaaki. / Positional cloning of silkworm white egg 2 (w-2) locus shows functional conservation and diversification of ABC transporters for pigmentation in insects. In: Genes to Cells. 2011 ; Vol. 16, No. 4. pp. 331-342.
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