Positions of disulfide bonds in rye (secale cereale) seed chitinase-a

Takeshi Yamagami, Gunki Funatsu, Masatsune Ishiguro

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The positions of disulfide bonds of rye seed chitinase-a (RSC-a) were identified by the isolation of disulfide-containing peptides produced with enzymatic and/or chemical cleavages of RSC-a, followed by sequencing them. An unequivocal assignment of disulfide bonds in this enzyme was as follows: Cys3-Cys18, Cys12-Cys24, Cys15-Cys42, Cys17-Cys31, and Cys35-Cys39 in the chitin-binding domain (CB domain), Cys82-Cys144, Cys156-Cys164, and Cys282-Cys295 in the catalytic domain (Cat domain), and Cys263 was a free form.

Original languageEnglish
Pages (from-to)1313-1316
Number of pages4
JournalBioscience, Biotechnology and Biochemistry
Volume64
Issue number6
DOIs
Publication statusPublished - Jan 1 2000

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Chitinases
Disulfides
Seed
Seeds
Chitin
Catalytic Domain
Peptides
Enzymes
Secale

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

Cite this

Positions of disulfide bonds in rye (secale cereale) seed chitinase-a. / Yamagami, Takeshi; Funatsu, Gunki; Ishiguro, Masatsune.

In: Bioscience, Biotechnology and Biochemistry, Vol. 64, No. 6, 01.01.2000, p. 1313-1316.

Research output: Contribution to journalArticle

Yamagami, Takeshi ; Funatsu, Gunki ; Ishiguro, Masatsune. / Positions of disulfide bonds in rye (secale cereale) seed chitinase-a. In: Bioscience, Biotechnology and Biochemistry. 2000 ; Vol. 64, No. 6. pp. 1313-1316.
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