Positive and negative CD4+ thymocyte selection by a single MHC class II/Peptide ligand affected by its expression level in the thymus

Yoshinori Fukui, Tatsuro Ishimoto, Masanori Utsuyama, Takahiro Gyotoku, Takahiro Koga, Kazutaka Nakao, Katsuiku Hirokawa, Motoya Katsuki, Takehiko Sasazuki

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105 Citations (Scopus)

Abstract

The central event in thymic selection of T cells bearing αβ TCRs is their interaction with self-peptides bound to self-MHC molecules. With the use of transgenic mouse lines expressing a single peptide/MHC class II complex, we show that CD4+ T cells with the preferential usage of particular TCR Vαs and Vβs were selected to mature on this complex in lines with the lower expression, whereas such CD4+ T cells were eliminated in the thymus in a line with the relatively high expression. When a low expressing line was crossed with a high expressing line, the frequency of CD4+ T cells selected by this complex markedly decreased. Thus, these results suggest that a single peptide/MHC class II complex, being affected by its cell surface density in the thymus, can serve as both positively and negatively selecting ligand in vivo.

Original languageEnglish
Pages (from-to)401-410
Number of pages10
JournalImmunity
Volume6
Issue number4
DOIs
Publication statusPublished - Apr 1 1997

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology
  • Infectious Diseases

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    Fukui, Y., Ishimoto, T., Utsuyama, M., Gyotoku, T., Koga, T., Nakao, K., Hirokawa, K., Katsuki, M., & Sasazuki, T. (1997). Positive and negative CD4+ thymocyte selection by a single MHC class II/Peptide ligand affected by its expression level in the thymus. Immunity, 6(4), 401-410. https://doi.org/10.1016/S1074-7613(00)80283-6