Positive-feedback-mode scanning electrochemical microscopy imaging of redox-active DNA-poly(1,4-benzoquinone) conjugate film deposited on carbon fiber electrode for micrometer-sized hybridization biosensor applications

Koji Nakano, Kaori Nakamura, Kaori Iwamoto, Nobuaki Soh, Toshihiko Imato

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Scanning electrochemical microscopy of DNA microdots deposited on carbon fiber microelectrodes (diameter, 33 μm) has been demonstrated. The microdots, which comprised quinone polymer matrices with capture probe (CP) DNA grafted onto them, can report hybridization events via changes in their electrochemical reactivity. Furthermore, the polymer matrices, even after conjugation with CP DNA, possess a certain degree of charge-transport capability and thus allow for positive-feedback-mode imaging. We have successfully obtained well-resolved micrometer-sized dot images (diameter, 60-100 μm) of the microelectrodes: they generate a considerable magnitude of current rise over 10 nA while they gave a current decrease, typically 1 nA, in responding the hybridization event at the CP DNA. The sensor response was found to fall a little larger than the background current (0.6-0.8 nA). However, the particular SECM measurement system represented good signal-to-noise ratio reliably allowing the detection of DNA hybridization feasible. Obtaining these results, we have concluded that the particular DNA-modified electrode with SECM detection should be useful for readout of DNA hybridization sensor coupled with a high-throughput-device such as DNA microarrays.

Original languageEnglish
Pages (from-to)113-118
Number of pages6
JournalJournal of Electroanalytical Chemistry
Volume628
Issue number1-2
DOIs
Publication statusPublished - Apr 1 2009

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Biosensors
Carbon fibers
Microscopic examination
DNA
Scanning
DNA Probes
Feedback
Imaging techniques
Electrodes
Microelectrodes
Polymer matrix
Sensors
Microarrays
Charge transfer
Signal to noise ratio
Throughput
Oxidation-Reduction
benzoquinone
carbon fiber

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Chemical Engineering(all)
  • Electrochemistry

Cite this

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title = "Positive-feedback-mode scanning electrochemical microscopy imaging of redox-active DNA-poly(1,4-benzoquinone) conjugate film deposited on carbon fiber electrode for micrometer-sized hybridization biosensor applications",
abstract = "Scanning electrochemical microscopy of DNA microdots deposited on carbon fiber microelectrodes (diameter, 33 μm) has been demonstrated. The microdots, which comprised quinone polymer matrices with capture probe (CP) DNA grafted onto them, can report hybridization events via changes in their electrochemical reactivity. Furthermore, the polymer matrices, even after conjugation with CP DNA, possess a certain degree of charge-transport capability and thus allow for positive-feedback-mode imaging. We have successfully obtained well-resolved micrometer-sized dot images (diameter, 60-100 μm) of the microelectrodes: they generate a considerable magnitude of current rise over 10 nA while they gave a current decrease, typically 1 nA, in responding the hybridization event at the CP DNA. The sensor response was found to fall a little larger than the background current (0.6-0.8 nA). However, the particular SECM measurement system represented good signal-to-noise ratio reliably allowing the detection of DNA hybridization feasible. Obtaining these results, we have concluded that the particular DNA-modified electrode with SECM detection should be useful for readout of DNA hybridization sensor coupled with a high-throughput-device such as DNA microarrays.",
author = "Koji Nakano and Kaori Nakamura and Kaori Iwamoto and Nobuaki Soh and Toshihiko Imato",
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AU - Nakano, Koji

AU - Nakamura, Kaori

AU - Iwamoto, Kaori

AU - Soh, Nobuaki

AU - Imato, Toshihiko

PY - 2009/4/1

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N2 - Scanning electrochemical microscopy of DNA microdots deposited on carbon fiber microelectrodes (diameter, 33 μm) has been demonstrated. The microdots, which comprised quinone polymer matrices with capture probe (CP) DNA grafted onto them, can report hybridization events via changes in their electrochemical reactivity. Furthermore, the polymer matrices, even after conjugation with CP DNA, possess a certain degree of charge-transport capability and thus allow for positive-feedback-mode imaging. We have successfully obtained well-resolved micrometer-sized dot images (diameter, 60-100 μm) of the microelectrodes: they generate a considerable magnitude of current rise over 10 nA while they gave a current decrease, typically 1 nA, in responding the hybridization event at the CP DNA. The sensor response was found to fall a little larger than the background current (0.6-0.8 nA). However, the particular SECM measurement system represented good signal-to-noise ratio reliably allowing the detection of DNA hybridization feasible. Obtaining these results, we have concluded that the particular DNA-modified electrode with SECM detection should be useful for readout of DNA hybridization sensor coupled with a high-throughput-device such as DNA microarrays.

AB - Scanning electrochemical microscopy of DNA microdots deposited on carbon fiber microelectrodes (diameter, 33 μm) has been demonstrated. The microdots, which comprised quinone polymer matrices with capture probe (CP) DNA grafted onto them, can report hybridization events via changes in their electrochemical reactivity. Furthermore, the polymer matrices, even after conjugation with CP DNA, possess a certain degree of charge-transport capability and thus allow for positive-feedback-mode imaging. We have successfully obtained well-resolved micrometer-sized dot images (diameter, 60-100 μm) of the microelectrodes: they generate a considerable magnitude of current rise over 10 nA while they gave a current decrease, typically 1 nA, in responding the hybridization event at the CP DNA. The sensor response was found to fall a little larger than the background current (0.6-0.8 nA). However, the particular SECM measurement system represented good signal-to-noise ratio reliably allowing the detection of DNA hybridization feasible. Obtaining these results, we have concluded that the particular DNA-modified electrode with SECM detection should be useful for readout of DNA hybridization sensor coupled with a high-throughput-device such as DNA microarrays.

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