Abstract
Most T cells develop through the thymus, where they undergo positive and negative selection. Some peripheral T cells are known to develop in the absence of thymus, but there is insufficient information about their selection. To analyze the selection of extrathymically developed T cells, we reconstituted thymectomized male or female recipient mice with bone marrow cells of mice transgenic for male H-Y antigen-specific T cell receptor (TCR). It was revealed that the T cells bearing self-antigen-specific TCR were not deleted in thymectomized male recipients. More importantly, the absence of H- Y antigen-specific T cells in thymectomized female recipients suggests positive selection of extrathymically developed T cells by the self-antigen. The extrathymically developed T cells in male mice expressed interleukin (IL)-2 receptor β chain (IL-2Rβ) and intermediate levels of CD3 (CD3(int)) but were natural killer cell (NK) 1.1-. They rapidly produced interferon γ but not IL-4 after TCR cross-linking. Furthermore, a similar pattern of cytokine production was observed in CD3(int)IL-2RB+NK1.1- cells in normal mice which have been shown to develop extrathymically. These results suggest that extrathymically developed CD3(int)IL-2Rβ+NK1.1- cells in normal mice are also positively selected by self-antigens.
| Original language | English |
|---|---|
| Pages (from-to) | 779-784 |
| Number of pages | 6 |
| Journal | Journal of Experimental Medicine |
| Volume | 188 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - Aug 17 1998 |
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All Science Journal Classification (ASJC) codes
- Immunology and Allergy
- Immunology
Cite this
Positive selection of extrathymically developed T cells by self-antigens. / Yamada, Hisakata; Ninomiya, Toshiharu; Hashimoto, Asako; Tamada, Koji; Takimoto, Hiroaki; Nomoto, Kikuo.
In: Journal of Experimental Medicine, Vol. 188, No. 4, 17.08.1998, p. 779-784.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Positive selection of extrathymically developed T cells by self-antigens
AU - Yamada, Hisakata
AU - Ninomiya, Toshiharu
AU - Hashimoto, Asako
AU - Tamada, Koji
AU - Takimoto, Hiroaki
AU - Nomoto, Kikuo
PY - 1998/8/17
Y1 - 1998/8/17
N2 - Most T cells develop through the thymus, where they undergo positive and negative selection. Some peripheral T cells are known to develop in the absence of thymus, but there is insufficient information about their selection. To analyze the selection of extrathymically developed T cells, we reconstituted thymectomized male or female recipient mice with bone marrow cells of mice transgenic for male H-Y antigen-specific T cell receptor (TCR). It was revealed that the T cells bearing self-antigen-specific TCR were not deleted in thymectomized male recipients. More importantly, the absence of H- Y antigen-specific T cells in thymectomized female recipients suggests positive selection of extrathymically developed T cells by the self-antigen. The extrathymically developed T cells in male mice expressed interleukin (IL)-2 receptor β chain (IL-2Rβ) and intermediate levels of CD3 (CD3(int)) but were natural killer cell (NK) 1.1-. They rapidly produced interferon γ but not IL-4 after TCR cross-linking. Furthermore, a similar pattern of cytokine production was observed in CD3(int)IL-2RB+NK1.1- cells in normal mice which have been shown to develop extrathymically. These results suggest that extrathymically developed CD3(int)IL-2Rβ+NK1.1- cells in normal mice are also positively selected by self-antigens.
AB - Most T cells develop through the thymus, where they undergo positive and negative selection. Some peripheral T cells are known to develop in the absence of thymus, but there is insufficient information about their selection. To analyze the selection of extrathymically developed T cells, we reconstituted thymectomized male or female recipient mice with bone marrow cells of mice transgenic for male H-Y antigen-specific T cell receptor (TCR). It was revealed that the T cells bearing self-antigen-specific TCR were not deleted in thymectomized male recipients. More importantly, the absence of H- Y antigen-specific T cells in thymectomized female recipients suggests positive selection of extrathymically developed T cells by the self-antigen. The extrathymically developed T cells in male mice expressed interleukin (IL)-2 receptor β chain (IL-2Rβ) and intermediate levels of CD3 (CD3(int)) but were natural killer cell (NK) 1.1-. They rapidly produced interferon γ but not IL-4 after TCR cross-linking. Furthermore, a similar pattern of cytokine production was observed in CD3(int)IL-2RB+NK1.1- cells in normal mice which have been shown to develop extrathymically. These results suggest that extrathymically developed CD3(int)IL-2Rβ+NK1.1- cells in normal mice are also positively selected by self-antigens.
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UR - http://www.scopus.com/inward/citedby.url?scp=0032541387&partnerID=8YFLogxK
U2 - 10.1084/jem.188.4.779
DO - 10.1084/jem.188.4.779
M3 - Article
C2 - 9705960
AN - SCOPUS:0032541387
VL - 188
SP - 779
EP - 784
JO - Journal of Experimental Medicine
JF - Journal of Experimental Medicine
SN - 0022-1007
IS - 4
ER -