Positive selection of extrathymically developed T cells by self-antigens

Hisakata Yamada, Toshiharu Ninomiya, Asako Hashimoto, Koji Tamada, Hiroaki Takimoto, Kikuo Nomoto

Research output: Contribution to journalArticle

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Abstract

Most T cells develop through the thymus, where they undergo positive and negative selection. Some peripheral T cells are known to develop in the absence of thymus, but there is insufficient information about their selection. To analyze the selection of extrathymically developed T cells, we reconstituted thymectomized male or female recipient mice with bone marrow cells of mice transgenic for male H-Y antigen-specific T cell receptor (TCR). It was revealed that the T cells bearing self-antigen-specific TCR were not deleted in thymectomized male recipients. More importantly, the absence of H- Y antigen-specific T cells in thymectomized female recipients suggests positive selection of extrathymically developed T cells by the self-antigen. The extrathymically developed T cells in male mice expressed interleukin (IL)-2 receptor β chain (IL-2Rβ) and intermediate levels of CD3 (CD3(int)) but were natural killer cell (NK) 1.1-. They rapidly produced interferon γ but not IL-4 after TCR cross-linking. Furthermore, a similar pattern of cytokine production was observed in CD3(int)IL-2RB+NK1.1- cells in normal mice which have been shown to develop extrathymically. These results suggest that extrathymically developed CD3(int)IL-2Rβ+NK1.1- cells in normal mice are also positively selected by self-antigens.

Original languageEnglish
Pages (from-to)779-784
Number of pages6
JournalJournal of Experimental Medicine
Volume188
Issue number4
DOIs
Publication statusPublished - Aug 17 1998

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Autoantigens
T-Lymphocytes
T-Cell Antigen Receptor
H-Y Antigen
Interleukins
Thymus Gland
Interleukin-2 Receptors
Natural Killer Cells
Bone Marrow Cells
Interleukin-4
Interferons
Transgenic Mice
Cytokines

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Cite this

Positive selection of extrathymically developed T cells by self-antigens. / Yamada, Hisakata; Ninomiya, Toshiharu; Hashimoto, Asako; Tamada, Koji; Takimoto, Hiroaki; Nomoto, Kikuo.

In: Journal of Experimental Medicine, Vol. 188, No. 4, 17.08.1998, p. 779-784.

Research output: Contribution to journalArticle

Yamada, Hisakata ; Ninomiya, Toshiharu ; Hashimoto, Asako ; Tamada, Koji ; Takimoto, Hiroaki ; Nomoto, Kikuo. / Positive selection of extrathymically developed T cells by self-antigens. In: Journal of Experimental Medicine. 1998 ; Vol. 188, No. 4. pp. 779-784.
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