Possible association between higher β-catenin mRNA expression and mutated β-catenin in sporadic desmoid tumors

Real-time semiquantitative assay by TaqMan polymerase chain reaction

Tsuyoshi Saito, Yoshinao Oda, Kenichi Kawaguchi, Kazuhiro Tanaka, Shuichi Matsuda, Sadafumi Tamiya, Yukihide Iwamoto, Masazumi Tsuneyoshi

Research output: Contribution to journalArticle

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Abstract

We screened for genetic alterations of adenomatous polyposis coli (APC) and β-catenin genes in 17 frozen specimens from 12 cases of sporadic desmoid tumors and then subdivided these cases into two groups according to the results of mutational analysis. We further examined mRNA expression of β-catenin and cyclin D1 by TaqMan PCR and compared the mRNA expression within both groups. Single-strand conformation polymorphism analysis followed by DNA direct sequencing revealed β-catenin mutation in 3 of 12 cases (6 of 17 specimens), whereas no APC missense mutations in the mutation cluster region were found. TaqMan PCR revealed extremely higher mRNA expression of β-catenin and cyclin D1 in desmoid tumors, compared with those of normal skeletal muscles. In the β-catenin mutated group, cyclin D1 mRNA expression was significantly higher than that of the β-catenin wild-type group (p = 0.0120, Mann-Whitney U test). In addition, in the β-catenin mutated group, β-catenin mRNA expression was also significantly higher than that of the β-catenin wild-type group (p = 0.0036, Mann-Whitney U test). All cases of desmoid tumors showed detectable β-catenin nuclear expression immunohistochemically. These results suggest that a continuously elevated β-catenin protein level caused by the β-catenin mutation itself may have a stronger power that can transactivate transcription in vivo. Furthermore, the results provide a possible association between higher β-catenin mRNA expression and mutated β-catenin in sporadic desmoid tumors. This may suggest that the β-catenin gene may be up-regulated by mutated or continuously elevated β-catenin protein, that is, the β-catenin gene may also be one of the targeted genes in the APC-β-catenin-Tcf pathway.

Original languageEnglish
Pages (from-to)97-103
Number of pages7
JournalLaboratory Investigation
Volume82
Issue number1
DOIs
Publication statusPublished - Jan 1 2002

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Aggressive Fibromatosis
Catenins
Polymerase Chain Reaction
Messenger RNA
Neoplasms
Cyclin D1
APC Genes
Nonparametric Statistics
Mutation
Adenomatous Polyposis Coli

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine
  • Molecular Biology
  • Cell Biology

Cite this

Possible association between higher β-catenin mRNA expression and mutated β-catenin in sporadic desmoid tumors : Real-time semiquantitative assay by TaqMan polymerase chain reaction. / Saito, Tsuyoshi; Oda, Yoshinao; Kawaguchi, Kenichi; Tanaka, Kazuhiro; Matsuda, Shuichi; Tamiya, Sadafumi; Iwamoto, Yukihide; Tsuneyoshi, Masazumi.

In: Laboratory Investigation, Vol. 82, No. 1, 01.01.2002, p. 97-103.

Research output: Contribution to journalArticle

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