Possible binding sites for inositol 1,4,5-trisphosphate in macrophages

Toyohiro Ishimatsu, Yuichi Kimura, Tetsuro Ikebe, Koji Yamaguchi, Toshitaka Koga, Masato Hirata

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

We reported that an arylazide derivative of inositol 1,4,5-trisphosphate (InsP 3 ) caused irreversible InsP 3 -induced Ca 2+ release from saponin-permeabilized macrophages after photoirradiation(1). To determine the specific receptors for InsP 3 , presumably present on the endoplastic reticulum, we synthesized isotope-labeled arylazide derivatives of InsP 3 ; InsP 3 was coupled with p-azidobenzoyl [ 3 h]β -alanine InsP 3 -[ 3 h]ABβ A ) or p-[ 125 i]azidosalicyl β-alanine (InsP 3 -[ 125 I]ASβ A). We report here that three proteins may be associated with the Ca 2+ releasing mechanism, in photoirradiated saponin-permeabilized macrophages and in the microsomal fraction.

Original languageEnglish
Pages (from-to)1173-1180
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume155
Issue number3
DOIs
Publication statusPublished - Sep 30 1988

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Inositol 1,4,5-Trisphosphate
Macrophages
Saponins
Alanine
Binding Sites
Derivatives
Reticulum
Isotopes
Proteins

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Possible binding sites for inositol 1,4,5-trisphosphate in macrophages. / Ishimatsu, Toyohiro; Kimura, Yuichi; Ikebe, Tetsuro; Yamaguchi, Koji; Koga, Toshitaka; Hirata, Masato.

In: Biochemical and Biophysical Research Communications, Vol. 155, No. 3, 30.09.1988, p. 1173-1180.

Research output: Contribution to journalArticle

Ishimatsu, T, Kimura, Y, Ikebe, T, Yamaguchi, K, Koga, T & Hirata, M 1988, 'Possible binding sites for inositol 1,4,5-trisphosphate in macrophages', Biochemical and Biophysical Research Communications, vol. 155, no. 3, pp. 1173-1180. https://doi.org/10.1016/S0006-291X(88)81263-4
Ishimatsu, Toyohiro ; Kimura, Yuichi ; Ikebe, Tetsuro ; Yamaguchi, Koji ; Koga, Toshitaka ; Hirata, Masato. / Possible binding sites for inositol 1,4,5-trisphosphate in macrophages. In: Biochemical and Biophysical Research Communications. 1988 ; Vol. 155, No. 3. pp. 1173-1180.
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