Possible function of salivary gland epithelial cells as nonprofessional antigen-presenting cells in the development of Sjögren's syndrome

Shizuka Tsunawaki, Seiji Nakamura, Yukiko Ohyama, Masanori Sasaki, Akiko Ikebe-Hiroki, Akimitsu Hiraki, Tsutomu Kadena, Eiji Kawamura, Wataru Kumamaru, Masanori Shinohara, Kanemitsu Shirasuna

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Abstract

Objective. To explore the potential of salivary gland epithelial cells to act as nonprofessional antigen-presenting cells (APC) in the development of Sjögren's syndrome (SS). Methods. Expression of HLA-DR antigens, costimulatory molecules, and adhesion molecules on epithelial cells was immunohistochemically examined in labial salivary glands from patients with SS. An association with the expression of T cell derived cytokine messenger RNA (mRNA) was observed. The expression of these molecules was confirmed using cultured salivary gland epithelial cells. The ability of the salivary gland epithelial cells as nonprofessional APC was examined in a mixed culture system using the salivary gland epithelial cells and allogeneic lymphocytes. Results. Expression of HLA-DR antigens, CD80, intercellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule (VCAM), and E-selectin was immunohistochemically detected on duct cells from all patients; however, the expression of CD86 was limited to only some patients. Concomitant expression of CD80 on duct cells and Th1 cytokine mRNA, and CD86 on duct cells and Th2 cytokine mRNA, was observed. Interferon-γ (IFN-γ) induced the cultured salivary gland epithelial cells to express HLA class I antigens, HLA-DR antigens, CD80, and ICAM-1, while tumor necrosis factor-α (TNF-α) induced the expression of HLA class I antigens, CD80, CD86, and VCAM. Cultured salivary gland epithelial cells treated with either IFN-γ or TNF-α also caused allogeneic lymphocytes to proliferate. Conclusion. The ability of salivary gland epithelial cells to express HLA-DR antigens, costimulatory molecules, and adhesion molecules and thus to act as nonprofessional APC was suggested. CD80 and CD86 expression of these cells was also suggested to be involved in the activation of Th1 and Th2, respectively.

Original languageEnglish
Pages (from-to)1884-1896
Number of pages13
JournalJournal of Rheumatology
Volume29
Issue number9
Publication statusPublished - Sep 1 2002

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Antigen-Presenting Cells
Salivary Glands
Epithelial Cells
HLA-DR Antigens
Histocompatibility Antigens Class I
Vascular Cell Adhesion Molecule-1
Intercellular Adhesion Molecule-1
Cytokines
HLA Antigens
Messenger RNA
Interferons
Tumor Necrosis Factor-alpha
Lymphocytes
Th2 Cells
Th1 Cells
E-Selectin
Lip
T-Lymphocytes

All Science Journal Classification (ASJC) codes

  • Rheumatology
  • Immunology and Allergy
  • Immunology

Cite this

Possible function of salivary gland epithelial cells as nonprofessional antigen-presenting cells in the development of Sjögren's syndrome. / Tsunawaki, Shizuka; Nakamura, Seiji; Ohyama, Yukiko; Sasaki, Masanori; Ikebe-Hiroki, Akiko; Hiraki, Akimitsu; Kadena, Tsutomu; Kawamura, Eiji; Kumamaru, Wataru; Shinohara, Masanori; Shirasuna, Kanemitsu.

In: Journal of Rheumatology, Vol. 29, No. 9, 01.09.2002, p. 1884-1896.

Research output: Contribution to journalArticle

Tsunawaki, S, Nakamura, S, Ohyama, Y, Sasaki, M, Ikebe-Hiroki, A, Hiraki, A, Kadena, T, Kawamura, E, Kumamaru, W, Shinohara, M & Shirasuna, K 2002, 'Possible function of salivary gland epithelial cells as nonprofessional antigen-presenting cells in the development of Sjögren's syndrome', Journal of Rheumatology, vol. 29, no. 9, pp. 1884-1896.
Tsunawaki, Shizuka ; Nakamura, Seiji ; Ohyama, Yukiko ; Sasaki, Masanori ; Ikebe-Hiroki, Akiko ; Hiraki, Akimitsu ; Kadena, Tsutomu ; Kawamura, Eiji ; Kumamaru, Wataru ; Shinohara, Masanori ; Shirasuna, Kanemitsu. / Possible function of salivary gland epithelial cells as nonprofessional antigen-presenting cells in the development of Sjögren's syndrome. In: Journal of Rheumatology. 2002 ; Vol. 29, No. 9. pp. 1884-1896.
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AU - Ikebe-Hiroki, Akiko

AU - Hiraki, Akimitsu

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