Possible involvement of aiPLA2 in the phosphatidylserine-containing liposomes induced production of PGE2 and PGD2 in microglia

Fumiko Takayama; Zhou Wu; Hong Mei Ma; Ryo Okada; Yoshinori Hayashi; Hiroshi Nakanishi

doi:10.1016/j.jneuroim.2013.06.011

Possible involvement of aiPLA₂ in the phosphatidylserine-containing liposomes induced production of PGE₂ and PGD₂ in microglia

Fumiko Takayama, Zhou Wu, Hong Mei Ma, Ryo Okada, Yoshinori Hayashi, Hiroshi Nakanishi

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

Liposomes containing phosphatidylserine (PSL) produce PGE₂ after being phagocytosed by microglia, but the precise underlying mechanism behind it still remains unclear. Here, we showed that liposomes consisting of phosphatidylserine and lysophosphatidylcholine, a lipolysis product of phosphatidylcholine by PLA₂, were phagocytosed by microglia, but failed to induce secretion of PGE₂. Furthermore, PSL-induced PGE₂ secretion was significantly inhibited by MJ33, an aiPLA₂ inhibitor, but not by AACOCF₃, a cPLA₂ inhibitor. PSL also produced PGD₂ and 15d-PGJ₂ in microglia. We thus hypothesize that free arachidonic acid is supplied through aiPLA₂-mediated lipolysis of phagocytosed phosphatidylcholine, leading to the production of PGH₂ and its downstream metabolites.

Original language	English
Pages (from-to)	121-124
Number of pages	4
Journal	Journal of Neuroimmunology
Volume	262
Issue number	1-2
DOIs	https://doi.org/10.1016/j.jneuroim.2013.06.011
Publication status	Published - 2013

All Science Journal Classification (ASJC) codes

Immunology and Allergy
Immunology
Neurology
Clinical Neurology

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10.1016/j.jneuroim.2013.06.011

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@article{c65a001636a747abbd33b7a3b9c1f6c9,

title = "Possible involvement of aiPLA2 in the phosphatidylserine-containing liposomes induced production of PGE2 and PGD2 in microglia",

abstract = "Liposomes containing phosphatidylserine (PSL) produce PGE2 after being phagocytosed by microglia, but the precise underlying mechanism behind it still remains unclear. Here, we showed that liposomes consisting of phosphatidylserine and lysophosphatidylcholine, a lipolysis product of phosphatidylcholine by PLA2, were phagocytosed by microglia, but failed to induce secretion of PGE2. Furthermore, PSL-induced PGE2 secretion was significantly inhibited by MJ33, an aiPLA2 inhibitor, but not by AACOCF3, a cPLA2 inhibitor. PSL also produced PGD2 and 15d-PGJ2 in microglia. We thus hypothesize that free arachidonic acid is supplied through aiPLA2-mediated lipolysis of phagocytosed phosphatidylcholine, leading to the production of PGH2 and its downstream metabolites.",

author = "Fumiko Takayama and Zhou Wu and Ma, {Hong Mei} and Ryo Okada and Yoshinori Hayashi and Hiroshi Nakanishi",

note = "Funding Information: This study was supported in part by Grants-in-Aid for Scientific Research from the Ministry of Education, Science, and Culture of Japan (Nos. 24390416 and 24659827 to H.N., No. 24592802 to Z.W.).",

year = "2013",

doi = "10.1016/j.jneuroim.2013.06.011",

language = "English",

volume = "262",

pages = "121--124",

journal = "Advances in Neuroimmunology",

issn = "0165-5728",

publisher = "Elsevier",

number = "1-2",

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TY - JOUR

T1 - Possible involvement of aiPLA2 in the phosphatidylserine-containing liposomes induced production of PGE2 and PGD2 in microglia

AU - Takayama, Fumiko

AU - Wu, Zhou

AU - Ma, Hong Mei

AU - Okada, Ryo

AU - Hayashi, Yoshinori

AU - Nakanishi, Hiroshi

N1 - Funding Information: This study was supported in part by Grants-in-Aid for Scientific Research from the Ministry of Education, Science, and Culture of Japan (Nos. 24390416 and 24659827 to H.N., No. 24592802 to Z.W.).

PY - 2013

Y1 - 2013

N2 - Liposomes containing phosphatidylserine (PSL) produce PGE2 after being phagocytosed by microglia, but the precise underlying mechanism behind it still remains unclear. Here, we showed that liposomes consisting of phosphatidylserine and lysophosphatidylcholine, a lipolysis product of phosphatidylcholine by PLA2, were phagocytosed by microglia, but failed to induce secretion of PGE2. Furthermore, PSL-induced PGE2 secretion was significantly inhibited by MJ33, an aiPLA2 inhibitor, but not by AACOCF3, a cPLA2 inhibitor. PSL also produced PGD2 and 15d-PGJ2 in microglia. We thus hypothesize that free arachidonic acid is supplied through aiPLA2-mediated lipolysis of phagocytosed phosphatidylcholine, leading to the production of PGH2 and its downstream metabolites.

AB - Liposomes containing phosphatidylserine (PSL) produce PGE2 after being phagocytosed by microglia, but the precise underlying mechanism behind it still remains unclear. Here, we showed that liposomes consisting of phosphatidylserine and lysophosphatidylcholine, a lipolysis product of phosphatidylcholine by PLA2, were phagocytosed by microglia, but failed to induce secretion of PGE2. Furthermore, PSL-induced PGE2 secretion was significantly inhibited by MJ33, an aiPLA2 inhibitor, but not by AACOCF3, a cPLA2 inhibitor. PSL also produced PGD2 and 15d-PGJ2 in microglia. We thus hypothesize that free arachidonic acid is supplied through aiPLA2-mediated lipolysis of phagocytosed phosphatidylcholine, leading to the production of PGH2 and its downstream metabolites.

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DO - 10.1016/j.jneuroim.2013.06.011

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AN - SCOPUS:84884167741

SN - 0165-5728

VL - 262

SP - 121

EP - 124

JO - Advances in Neuroimmunology

JF - Advances in Neuroimmunology

IS - 1-2

ER -

Possible involvement of aiPLA₂ in the phosphatidylserine-containing liposomes induced production of PGE₂ and PGD₂ in microglia

Abstract

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