Possible involvement of multidrug‐resistance‐associated protein (MRP) gene expression in spontaneous drug resistance to vincristine, etoposide and adriamycin in human glioma cells

Shuji Hasegawa, Ken Taniguchi, Akira Yokomizo, Akira Yokomizo, Mayumi Ono, Temaki Mori, Shigeaki Hori, Kimitoshi Kohno, Michihiko Kuwano

Research output: Contribution to journalArticle

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Abstract

The multidrug‐resistance phenotype in human tumors is partly associated with over‐expression of the 170 kDa‐P‐glycoprotein encoded by the multidrug‐resistance‐1 (MDRI) gene. Another related, but non‐P‐glycoprotein, multidrug‐resistance‐associated protein (MRP) gene encodes a 190 kDa membrane ATP‐binding protein. Glioblastoma multiforme is a highly malignant primary neoplasm of the central nervous system which is refractory to anti‐cancer chemotherapy, but the mechanism underlying this drug resistance is unknown. Out of glioma cell lines, 2, namely IN500 and T98G, which had elevated MRP mRNA levels, showed the highest resistance to multiple anti‐cancer agents such as etoposide, vincristine and adriamycin, and decreased intracellular accumulation of etoposide. In the remaining 5 cell lines, various degrees of sensitivity to adriamycin and etoposide appeared to correlate with their respective MRP mRNA levels. Our study proposes that MRP may be involved in spontaneous multidrug resistance in human gliomas.

Original languageEnglish
Pages (from-to)860-864
Number of pages5
JournalInternational Journal of Cancer
Volume58
Issue number6
DOIs
Publication statusPublished - Jan 1 1994

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Vincristine
Etoposide
Drug Resistance
Glioma
Doxorubicin
Gene Expression
Proteins
Cell Line
Central Nervous System Neoplasms
Messenger RNA
Multiple Drug Resistance
Glioblastoma
Antineoplastic Agents
Membrane Proteins
Phenotype
Drug Therapy
Genes
Neoplasms

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

Cite this

Possible involvement of multidrug‐resistance‐associated protein (MRP) gene expression in spontaneous drug resistance to vincristine, etoposide and adriamycin in human glioma cells. / Hasegawa, Shuji; Taniguchi, Ken; Yokomizo, Akira; Yokomizo, Akira; Ono, Mayumi; Mori, Temaki; Hori, Shigeaki; Kohno, Kimitoshi; Kuwano, Michihiko.

In: International Journal of Cancer, Vol. 58, No. 6, 01.01.1994, p. 860-864.

Research output: Contribution to journalArticle

Hasegawa, Shuji ; Taniguchi, Ken ; Yokomizo, Akira ; Yokomizo, Akira ; Ono, Mayumi ; Mori, Temaki ; Hori, Shigeaki ; Kohno, Kimitoshi ; Kuwano, Michihiko. / Possible involvement of multidrug‐resistance‐associated protein (MRP) gene expression in spontaneous drug resistance to vincristine, etoposide and adriamycin in human glioma cells. In: International Journal of Cancer. 1994 ; Vol. 58, No. 6. pp. 860-864.
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