Possible involvement of organic anion transporting polypeptide 1c1 in the photoperiodic response of gonads in birds

Nobuhiro Nakao, Tsuyoshi Takagi, Masayuki Iigo, Toshiro Tsukamoto, Shinobu Yasuo, Tomohiro Masuda, Tadashi Yanagisawa, Shizufumi Ebihara, Takashi Yoshimura

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

The photoperiodic response of the gonads requires T3, which is generated photoperiodically from T4 by type 2 iodothyronine deiodinase in the hypothalamus. Although thyroid hormones were long thought to traverse the plasma membrane by passive diffusion due to their lipophilic nature, it is now known that several organic anion transporting polypeptides (Oatp) transport thyroid hormones into target cells. In this study, we have used database searches to isolate DNA sequences encoding members of the chicken Oatp family and constructed a molecular phylogenetic tree. Comprehensive expression analyses using in situ hybridization revealed strong expression of cOatp1c1 and weak expression of cOatp1b1 in the ventro-lateral walls of basal tuberal hypothalamus, whereas expression of four genes (cOatp1a1, cOatp1b1, cOatp1c1, and cOatp3a2) was observed in the choroid plexus. Expression levels of all these genes in both regions were not different between short-day and long-day conditions. Functional expression of cOatp1c1 in Chinese hamster ovary cells revealed that cOatp1c1 is a highly specific transporter for T4 with an apparent Km of 6.8 nM and a Vmax of 1.50 pmol per milligram of protein per minute. These results suggest that cOatp1c1 could be involved in the thyroxine transport necessary for the avian photoperiodic response of the gonads.

Original languageEnglish
Pages (from-to)1067-1073
Number of pages7
JournalEndocrinology
Volume147
Issue number3
DOIs
Publication statusPublished - Mar 1 2006

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Gonads
Thyroid Hormones
Hypothalamus
Birds
Anions
Organic Anion Transporters
Iodide Peroxidase
Peptides
Choroid Plexus
Cricetulus
Thyroxine
In Situ Hybridization
Ovary
Chickens
Cell Membrane
Databases
Gene Expression
Genes
Proteins

All Science Journal Classification (ASJC) codes

  • Endocrinology

Cite this

Possible involvement of organic anion transporting polypeptide 1c1 in the photoperiodic response of gonads in birds. / Nakao, Nobuhiro; Takagi, Tsuyoshi; Iigo, Masayuki; Tsukamoto, Toshiro; Yasuo, Shinobu; Masuda, Tomohiro; Yanagisawa, Tadashi; Ebihara, Shizufumi; Yoshimura, Takashi.

In: Endocrinology, Vol. 147, No. 3, 01.03.2006, p. 1067-1073.

Research output: Contribution to journalArticle

Nakao, N, Takagi, T, Iigo, M, Tsukamoto, T, Yasuo, S, Masuda, T, Yanagisawa, T, Ebihara, S & Yoshimura, T 2006, 'Possible involvement of organic anion transporting polypeptide 1c1 in the photoperiodic response of gonads in birds', Endocrinology, vol. 147, no. 3, pp. 1067-1073. https://doi.org/10.1210/en.2005-1090
Nakao, Nobuhiro ; Takagi, Tsuyoshi ; Iigo, Masayuki ; Tsukamoto, Toshiro ; Yasuo, Shinobu ; Masuda, Tomohiro ; Yanagisawa, Tadashi ; Ebihara, Shizufumi ; Yoshimura, Takashi. / Possible involvement of organic anion transporting polypeptide 1c1 in the photoperiodic response of gonads in birds. In: Endocrinology. 2006 ; Vol. 147, No. 3. pp. 1067-1073.
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