Possible involvement of P2Y2 metabotropic receptors in ATP-induced transient receptor potential vanilloid receptor 1-mediated thermal hypersensitivity

Tomoko Moriyama, Tohko Iida, Kimiko Kobayashi, Tomohiro Higashi, Tetsuo Fukuoka, Hideki Tsumura, Catherine Leon, Noboru Suzuki, Kazuhide Inoue, Christian Gachet, Koichi Noguchi, Makoto Tominaga

Research output: Contribution to journalArticle

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Abstract

The capsaicin receptor transient receptor potential V1 (TRPV1; also known as vanilloid receptor 1) is a sensory neuron-specific ion channel that serves as a polymodal detector of pain-producing chemical and physical stimuli. It has been reported that extracellular ATP potentiates the TRPV1 currents evoked by capsaicin or protons and reduces the temperature threshold for its activation through metabotropic P2Y receptors in a PKC-dependent pathway, suggesting that TRPV1 activation could trigger the sensation of pain at normal body temperature in the presence of ATP. Here, we show that ATP-induced thermal hyperalgesia was abolished in mice lacking TRPV1, suggesting the functional interaction between ATP and TRPV1 at a behavioral level. However, thermal hyperalgesia was preserved in P2Y1 receptor-deficient mice. Patch-clamp analyses using mouse dorsal root ganglion neurons indicated the involvement of P2Y2 rather than P2Y1 receptors. Coexpression of TRPV1 mRNA with P2Y2 mRNA, but not P2Y1 mRNA, was determined in the rat lumbar DRG using in situ hybridization histochemistry. These data indicate the importance of metabotropic P2Y2 receptors in nociception through TRPV1.

Original languageEnglish
Pages (from-to)6058-6062
Number of pages5
JournalJournal of Neuroscience
Volume23
Issue number14
Publication statusPublished - Jul 9 2003

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Purinergic P2Y2 Receptors
Hypersensitivity
Hot Temperature
Adenosine Triphosphate
Purinergic P2Y1 Receptors
Hyperalgesia
Messenger RNA
TRPV Cation Channels
Pain
Vasopressin Receptors
Nociception
Diagnosis-Related Groups
Capsaicin
Spinal Ganglia
Sensory Receptor Cells
Body Temperature
Ion Channels
In Situ Hybridization
Protons
Neurons

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

Cite this

Moriyama, T., Iida, T., Kobayashi, K., Higashi, T., Fukuoka, T., Tsumura, H., ... Tominaga, M. (2003). Possible involvement of P2Y2 metabotropic receptors in ATP-induced transient receptor potential vanilloid receptor 1-mediated thermal hypersensitivity. Journal of Neuroscience, 23(14), 6058-6062.

Possible involvement of P2Y2 metabotropic receptors in ATP-induced transient receptor potential vanilloid receptor 1-mediated thermal hypersensitivity. / Moriyama, Tomoko; Iida, Tohko; Kobayashi, Kimiko; Higashi, Tomohiro; Fukuoka, Tetsuo; Tsumura, Hideki; Leon, Catherine; Suzuki, Noboru; Inoue, Kazuhide; Gachet, Christian; Noguchi, Koichi; Tominaga, Makoto.

In: Journal of Neuroscience, Vol. 23, No. 14, 09.07.2003, p. 6058-6062.

Research output: Contribution to journalArticle

Moriyama, T, Iida, T, Kobayashi, K, Higashi, T, Fukuoka, T, Tsumura, H, Leon, C, Suzuki, N, Inoue, K, Gachet, C, Noguchi, K & Tominaga, M 2003, 'Possible involvement of P2Y2 metabotropic receptors in ATP-induced transient receptor potential vanilloid receptor 1-mediated thermal hypersensitivity', Journal of Neuroscience, vol. 23, no. 14, pp. 6058-6062.
Moriyama, Tomoko ; Iida, Tohko ; Kobayashi, Kimiko ; Higashi, Tomohiro ; Fukuoka, Tetsuo ; Tsumura, Hideki ; Leon, Catherine ; Suzuki, Noboru ; Inoue, Kazuhide ; Gachet, Christian ; Noguchi, Koichi ; Tominaga, Makoto. / Possible involvement of P2Y2 metabotropic receptors in ATP-induced transient receptor potential vanilloid receptor 1-mediated thermal hypersensitivity. In: Journal of Neuroscience. 2003 ; Vol. 23, No. 14. pp. 6058-6062.
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