Possible involvement of protein phosphorylation in the wound-responsive expression of Arabidopsis plastid ω-3 fatty acid desaturase gene

Hiroaki Kodama, Takumi Nishiuchi, Shigemi Seo, Yuko Ohashi, Koh Iba

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The plastid ω-3 fatty acid desaturase (FAD7) catalyzes the conversion of linoleic acid to linolenic acid. Wounding enhances the expression of the FAD7 gene in leaves and induces its expression in stems and roots. The wound- induced expression of the FAD7 promoter was investigated in transgenic tobacco plants carrying the - 825 Arabidopsis FAD7 promoter::β-glucuronidase (GUS) fusion gene. The protein kinase inhibitor, staurosporine, and the protein phosphatase inhibitor, calyculin A, suppressed the wound induction of the FAD7 gene in stems. A tobacco mitogen-activated protein kinase (WIPK) was rapidly activated upon wounding not only in leaves but also in stems and roots, indicating that WIPK probably mediates the wound signals in most vegetative organs. The FAD7 promoter::GUS fusion gene was introduced into the transgenic tobacco plants in which the wipk gene was expressed constitutively at a high level or into the transgenic plants in which the wipk gene was suppressed possibly due to the transgene-induced gene silencing. The wound- induced expression of the FAD7 gene in stems was enhanced in the former transgenic tobacco plants and suppressed in the latter plants. These results suggest that the wound activation of the FAD7 promoter depends on both protein phosphorylation and dephosphorylation events especially in stems, and also that WIPK is involved in such signaling cascades. (C) 2000 Elsevier Science Ireland Ltd.

Original languageEnglish
Pages (from-to)153-160
Number of pages8
JournalPlant Science
Volume155
Issue number2
DOIs
Publication statusPublished - Jun 29 2000

Fingerprint

Fatty Acid Desaturases
stearoyl-CoA desaturase
Plastids
protein phosphorylation
plant damage
Arabidopsis
Genetically Modified Plants
plastids
Phosphorylation
Tobacco
tobacco
stems
promoter regions
Wounds and Injuries
gene fusion
Genes
Gene Fusion
Glucuronidase
genetically modified organisms
Proteins

All Science Journal Classification (ASJC) codes

  • Genetics
  • Agronomy and Crop Science
  • Plant Science

Cite this

Possible involvement of protein phosphorylation in the wound-responsive expression of Arabidopsis plastid ω-3 fatty acid desaturase gene. / Kodama, Hiroaki; Nishiuchi, Takumi; Seo, Shigemi; Ohashi, Yuko; Iba, Koh.

In: Plant Science, Vol. 155, No. 2, 29.06.2000, p. 153-160.

Research output: Contribution to journalArticle

Kodama, Hiroaki ; Nishiuchi, Takumi ; Seo, Shigemi ; Ohashi, Yuko ; Iba, Koh. / Possible involvement of protein phosphorylation in the wound-responsive expression of Arabidopsis plastid ω-3 fatty acid desaturase gene. In: Plant Science. 2000 ; Vol. 155, No. 2. pp. 153-160.
@article{fb831e85319f4203ae60032445924a94,
title = "Possible involvement of protein phosphorylation in the wound-responsive expression of Arabidopsis plastid ω-3 fatty acid desaturase gene",
abstract = "The plastid ω-3 fatty acid desaturase (FAD7) catalyzes the conversion of linoleic acid to linolenic acid. Wounding enhances the expression of the FAD7 gene in leaves and induces its expression in stems and roots. The wound- induced expression of the FAD7 promoter was investigated in transgenic tobacco plants carrying the - 825 Arabidopsis FAD7 promoter::β-glucuronidase (GUS) fusion gene. The protein kinase inhibitor, staurosporine, and the protein phosphatase inhibitor, calyculin A, suppressed the wound induction of the FAD7 gene in stems. A tobacco mitogen-activated protein kinase (WIPK) was rapidly activated upon wounding not only in leaves but also in stems and roots, indicating that WIPK probably mediates the wound signals in most vegetative organs. The FAD7 promoter::GUS fusion gene was introduced into the transgenic tobacco plants in which the wipk gene was expressed constitutively at a high level or into the transgenic plants in which the wipk gene was suppressed possibly due to the transgene-induced gene silencing. The wound- induced expression of the FAD7 gene in stems was enhanced in the former transgenic tobacco plants and suppressed in the latter plants. These results suggest that the wound activation of the FAD7 promoter depends on both protein phosphorylation and dephosphorylation events especially in stems, and also that WIPK is involved in such signaling cascades. (C) 2000 Elsevier Science Ireland Ltd.",
author = "Hiroaki Kodama and Takumi Nishiuchi and Shigemi Seo and Yuko Ohashi and Koh Iba",
year = "2000",
month = "6",
day = "29",
doi = "10.1016/S0168-9452(00)00210-7",
language = "English",
volume = "155",
pages = "153--160",
journal = "Plant Science",
issn = "0168-9452",
publisher = "Elsevier Ireland Ltd",
number = "2",

}

TY - JOUR

T1 - Possible involvement of protein phosphorylation in the wound-responsive expression of Arabidopsis plastid ω-3 fatty acid desaturase gene

AU - Kodama, Hiroaki

AU - Nishiuchi, Takumi

AU - Seo, Shigemi

AU - Ohashi, Yuko

AU - Iba, Koh

PY - 2000/6/29

Y1 - 2000/6/29

N2 - The plastid ω-3 fatty acid desaturase (FAD7) catalyzes the conversion of linoleic acid to linolenic acid. Wounding enhances the expression of the FAD7 gene in leaves and induces its expression in stems and roots. The wound- induced expression of the FAD7 promoter was investigated in transgenic tobacco plants carrying the - 825 Arabidopsis FAD7 promoter::β-glucuronidase (GUS) fusion gene. The protein kinase inhibitor, staurosporine, and the protein phosphatase inhibitor, calyculin A, suppressed the wound induction of the FAD7 gene in stems. A tobacco mitogen-activated protein kinase (WIPK) was rapidly activated upon wounding not only in leaves but also in stems and roots, indicating that WIPK probably mediates the wound signals in most vegetative organs. The FAD7 promoter::GUS fusion gene was introduced into the transgenic tobacco plants in which the wipk gene was expressed constitutively at a high level or into the transgenic plants in which the wipk gene was suppressed possibly due to the transgene-induced gene silencing. The wound- induced expression of the FAD7 gene in stems was enhanced in the former transgenic tobacco plants and suppressed in the latter plants. These results suggest that the wound activation of the FAD7 promoter depends on both protein phosphorylation and dephosphorylation events especially in stems, and also that WIPK is involved in such signaling cascades. (C) 2000 Elsevier Science Ireland Ltd.

AB - The plastid ω-3 fatty acid desaturase (FAD7) catalyzes the conversion of linoleic acid to linolenic acid. Wounding enhances the expression of the FAD7 gene in leaves and induces its expression in stems and roots. The wound- induced expression of the FAD7 promoter was investigated in transgenic tobacco plants carrying the - 825 Arabidopsis FAD7 promoter::β-glucuronidase (GUS) fusion gene. The protein kinase inhibitor, staurosporine, and the protein phosphatase inhibitor, calyculin A, suppressed the wound induction of the FAD7 gene in stems. A tobacco mitogen-activated protein kinase (WIPK) was rapidly activated upon wounding not only in leaves but also in stems and roots, indicating that WIPK probably mediates the wound signals in most vegetative organs. The FAD7 promoter::GUS fusion gene was introduced into the transgenic tobacco plants in which the wipk gene was expressed constitutively at a high level or into the transgenic plants in which the wipk gene was suppressed possibly due to the transgene-induced gene silencing. The wound- induced expression of the FAD7 gene in stems was enhanced in the former transgenic tobacco plants and suppressed in the latter plants. These results suggest that the wound activation of the FAD7 promoter depends on both protein phosphorylation and dephosphorylation events especially in stems, and also that WIPK is involved in such signaling cascades. (C) 2000 Elsevier Science Ireland Ltd.

UR - http://www.scopus.com/inward/record.url?scp=0034729753&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034729753&partnerID=8YFLogxK

U2 - 10.1016/S0168-9452(00)00210-7

DO - 10.1016/S0168-9452(00)00210-7

M3 - Article

AN - SCOPUS:0034729753

VL - 155

SP - 153

EP - 160

JO - Plant Science

JF - Plant Science

SN - 0168-9452

IS - 2

ER -