TY - JOUR
T1 - Possible involvement of soluble B7-H4 in T cell-mediated inflammatory immune responses
AU - Kamimura, Yosuke
AU - Kobori, Hiroko
AU - Piao, Jinhua
AU - Hashiguchi, Masaaki
AU - Matsumoto, Koichiro
AU - Hirose, Sachiko
AU - Azuma, Miyuki
N1 - Funding Information:
We thank Drs. T. Kitamura and H. Yagita for providing the vectors and retrovirus system and anti-Fas ligand mAb, respectively. This paper was supported in part by Grants-in-Aid from the Ministry of Education, Culture, Sports, Science and Technology of Japan .
PY - 2009/11/13
Y1 - 2009/11/13
N2 - B7-H4, a newly identified B7 family molecule, is reported to regulate T cell activation. However, the expression and function of B7-H4 remain controversial. Here, we demonstrated that B7-H4 expression in immune cells was undetectable at both the transcription and cell-surface protein levels. B7-H4 transfectants augmented anti-CD3 mAb-induced re-directed cytotoxicity and this was inhibited by anti-B7-H4 mAb. In a hapten-induced contact hypersensitivity model, treatment with anti-B7-H4 mAb at sensitization, but not at challenge, efficiently suppressed the ear swelling and CD8+ T cell activation assessed by CD25 expression and IFN-γ production. We found that cells expressing B7-H4 secreted soluble B7-H4 and the serum B7-H4 level increased with disease progression in lupus-prone and collagen-induced arthritis autoimmune mice and after the antigen challenge in allergic inflammatory diseases. Our results suggest a different action of B7-H4 in T cell-mediated inflammatory responses and the possible involvement of soluble B7-H4 in inflammatory immune responses.
AB - B7-H4, a newly identified B7 family molecule, is reported to regulate T cell activation. However, the expression and function of B7-H4 remain controversial. Here, we demonstrated that B7-H4 expression in immune cells was undetectable at both the transcription and cell-surface protein levels. B7-H4 transfectants augmented anti-CD3 mAb-induced re-directed cytotoxicity and this was inhibited by anti-B7-H4 mAb. In a hapten-induced contact hypersensitivity model, treatment with anti-B7-H4 mAb at sensitization, but not at challenge, efficiently suppressed the ear swelling and CD8+ T cell activation assessed by CD25 expression and IFN-γ production. We found that cells expressing B7-H4 secreted soluble B7-H4 and the serum B7-H4 level increased with disease progression in lupus-prone and collagen-induced arthritis autoimmune mice and after the antigen challenge in allergic inflammatory diseases. Our results suggest a different action of B7-H4 in T cell-mediated inflammatory responses and the possible involvement of soluble B7-H4 in inflammatory immune responses.
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U2 - 10.1016/j.bbrc.2009.08.144
DO - 10.1016/j.bbrc.2009.08.144
M3 - Article
C2 - 19723502
AN - SCOPUS:70349325495
SN - 0006-291X
VL - 389
SP - 349
EP - 353
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -