Post-ischemic treatment with Toki-shakuyaku-san (Tang-Gui-Shao-Yao-San) prevents the impairment of spatial memory induced by repeated cerebral ischemia in rats

Fengling Pu, Kenichi Mishima, Nobuaki Egashira, Yuki Akiyoshi, An xin Liu, Kazunori Sano, Keiichi Irie, Daisuke Ishibashi, Izzettin Hatip-Al-Khatib, Kiyo Iwasaki, Kouji Kurauchi, Katsunori Iwasaki, Michihiro Fujiwara

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23 Citations (Scopus)

Abstract

Previously we have reported that Toki-shakuyaku-san (TSS) ameliorated the impairment of spatial memory induced by single cerebral ischemia (1 × 10 minutes) and scopolamine, a muscarinic receptor antagonist. In this experiment, we studied the effect of TSS on repeated cerebral ischemia (2 × 10 minutes, 1-hour interval) induced impairment of spatial memory and neuronal injury in rats. The 8-day post-ischemic treatment with TSS (30-300 mg/kg) was administered p.o. once per day. TSS dose-dependently prevented the impairment of spatial memory, neuronal death and TUNEL positive cells induced by repeated cerebral ischemia. In order to determine the mechanism of TSS, we also studied the effect of TSS on GluR2 mRNA, one of the glutamate α-amino-3- hydroxy-5-methyl-4-isoxazole (AMPA) receptor subunits. Repeated cerebral ischemia significantly decreased GluR2 flop mRNA at 1 and 3 days after the occlusion. TSS (300 mg/kg) significantly suppressed the decrease in GluR2 flop at 3 days after repeated cerebral ischemia. These results suggested that the TSS has neuroprotective action which may be indirectly mediated by the AMPA receptor, and TSS may be beneficial for the treatment of cerebrovascular dementia.

Original languageEnglish
Pages (from-to)475-489
Number of pages15
JournalAmerican Journal of Chinese Medicine
Volume33
Issue number3
DOIs
Publication statusPublished - Jul 13 2005
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Complementary and alternative medicine

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    Pu, F., Mishima, K., Egashira, N., Akiyoshi, Y., Liu, A. X., Sano, K., Irie, K., Ishibashi, D., Hatip-Al-Khatib, I., Iwasaki, K., Kurauchi, K., Iwasaki, K., & Fujiwara, M. (2005). Post-ischemic treatment with Toki-shakuyaku-san (Tang-Gui-Shao-Yao-San) prevents the impairment of spatial memory induced by repeated cerebral ischemia in rats. American Journal of Chinese Medicine, 33(3), 475-489. https://doi.org/10.1142/S0192415X05003077