Post-translational modifications of the N-terminal tail of histone H3 in holocentric chromosomes of Bombyx mori

Hiroaki Mon, Makiko Izumi, Hitoshi Mitsunobu, Tsuneyuki Tatsuke, Kazuhiro Iiyama, Hiroyuki Jikuya, Jae Man Lee, Takahiro Kusakabe

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Abstract

Epigenetic information is encoded in post-translational modifications (PTMs) of histones. Various combinations of these marks contribute to the regulation of chromatin-templated DNA metabolisms. The histone code is gradually translated into biological responses in model organisms. However, in the silkworm, the modifications of histones with unique holocentric chromosomes have not yet been analyzed. TAU-PAGE analysis of the silkworm histone variants H2A, H2B, and H3, separated by RP-HPLC, suggested silkworm specific modification. Detailed mass spectrometry analyses of the peptides derived from the N-terminus of the silkworm H3.2 generated by glutamyl endopeptidase, lysyl endopeptidase, and trypsin digestions revealed global modifications around H3K9.

Original languageEnglish
Pages (from-to)902-908
Number of pages7
JournalInsect Biochemistry and Molecular Biology
Volume41
Issue number11
DOIs
Publication statusPublished - Nov 1 2011

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All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Insect Science

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