Postmortem interval estimation using the animal model of postmortem gas volume changes

Chika Iwamoto, Kenoki Ohuchida, Miki Okumura, Yosuke Usumoto, Junji Kishimoto, Masaharu Murata, Noriaki Ikeda, Makoto Hashizume

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

It is important to estimate the postmortem interval in forensic autopsy. Many methods to estimate the postmortem interval have been reported, and are typically associated with internal examination. However, there are issues such as rejection of autopsy by the family and a lack of forensic doctor in internal examination. Therefore, it is necessary to develop new methods, such as autopsy imaging, that can substitute for internal examination. Here, we first evaluated whether gas volume in the body increased with postmortem interval. Time-dependent X-ray CT imaging of euthanized Crl:CD (SD) rats (n = 3) was performed immediately after euthanasia and at seven subsequent time points up to 168 h (7 days) at 24-hour intervals. The data revealed that gas volume in the body increased in a time-dependent manner. Next, we reconstructed 3D images of isolated gas and calculated the gas volume using Amira software. In all cases, the volume of both portal venous gas and intestinal gas increased in a time-dependent manner. The volume of portal venous gas increased exponentially, while the volume of intestinal gas increased in a linearly with time. These data might be suggested that the postmortem gas volume change is one of indicators for estimating the postmortem interval. In addition, it would be possible to estimate more accurate postmortem interval by combining not only gas volume changes at the above two sites but also gas volume changes of the other sites such as heart cavities, kidney parenchyma, or abdominal aorta.

Original languageEnglish
Pages (from-to)66-70
Number of pages5
JournalLegal Medicine
Volume32
DOIs
Publication statusPublished - May 2018

Fingerprint

Animal Models
Gases
Autopsy
X Ray Computed Tomography
Euthanasia
Abdominal Aorta
Software
Kidney

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine
  • Issues, ethics and legal aspects

Cite this

Postmortem interval estimation using the animal model of postmortem gas volume changes. / Iwamoto, Chika; Ohuchida, Kenoki; Okumura, Miki; Usumoto, Yosuke; Kishimoto, Junji; Murata, Masaharu; Ikeda, Noriaki; Hashizume, Makoto.

In: Legal Medicine, Vol. 32, 05.2018, p. 66-70.

Research output: Contribution to journalArticle

@article{1ef4b97ac1884ea3b5c37a126002fae8,
title = "Postmortem interval estimation using the animal model of postmortem gas volume changes",
abstract = "It is important to estimate the postmortem interval in forensic autopsy. Many methods to estimate the postmortem interval have been reported, and are typically associated with internal examination. However, there are issues such as rejection of autopsy by the family and a lack of forensic doctor in internal examination. Therefore, it is necessary to develop new methods, such as autopsy imaging, that can substitute for internal examination. Here, we first evaluated whether gas volume in the body increased with postmortem interval. Time-dependent X-ray CT imaging of euthanized Crl:CD (SD) rats (n = 3) was performed immediately after euthanasia and at seven subsequent time points up to 168 h (7 days) at 24-hour intervals. The data revealed that gas volume in the body increased in a time-dependent manner. Next, we reconstructed 3D images of isolated gas and calculated the gas volume using Amira software. In all cases, the volume of both portal venous gas and intestinal gas increased in a time-dependent manner. The volume of portal venous gas increased exponentially, while the volume of intestinal gas increased in a linearly with time. These data might be suggested that the postmortem gas volume change is one of indicators for estimating the postmortem interval. In addition, it would be possible to estimate more accurate postmortem interval by combining not only gas volume changes at the above two sites but also gas volume changes of the other sites such as heart cavities, kidney parenchyma, or abdominal aorta.",
author = "Chika Iwamoto and Kenoki Ohuchida and Miki Okumura and Yosuke Usumoto and Junji Kishimoto and Masaharu Murata and Noriaki Ikeda and Makoto Hashizume",
year = "2018",
month = "5",
doi = "10.1016/j.legalmed.2017.12.010",
language = "English",
volume = "32",
pages = "66--70",
journal = "Legal Medicine",
issn = "1344-6223",
publisher = "Elsevier",

}

TY - JOUR

T1 - Postmortem interval estimation using the animal model of postmortem gas volume changes

AU - Iwamoto, Chika

AU - Ohuchida, Kenoki

AU - Okumura, Miki

AU - Usumoto, Yosuke

AU - Kishimoto, Junji

AU - Murata, Masaharu

AU - Ikeda, Noriaki

AU - Hashizume, Makoto

PY - 2018/5

Y1 - 2018/5

N2 - It is important to estimate the postmortem interval in forensic autopsy. Many methods to estimate the postmortem interval have been reported, and are typically associated with internal examination. However, there are issues such as rejection of autopsy by the family and a lack of forensic doctor in internal examination. Therefore, it is necessary to develop new methods, such as autopsy imaging, that can substitute for internal examination. Here, we first evaluated whether gas volume in the body increased with postmortem interval. Time-dependent X-ray CT imaging of euthanized Crl:CD (SD) rats (n = 3) was performed immediately after euthanasia and at seven subsequent time points up to 168 h (7 days) at 24-hour intervals. The data revealed that gas volume in the body increased in a time-dependent manner. Next, we reconstructed 3D images of isolated gas and calculated the gas volume using Amira software. In all cases, the volume of both portal venous gas and intestinal gas increased in a time-dependent manner. The volume of portal venous gas increased exponentially, while the volume of intestinal gas increased in a linearly with time. These data might be suggested that the postmortem gas volume change is one of indicators for estimating the postmortem interval. In addition, it would be possible to estimate more accurate postmortem interval by combining not only gas volume changes at the above two sites but also gas volume changes of the other sites such as heart cavities, kidney parenchyma, or abdominal aorta.

AB - It is important to estimate the postmortem interval in forensic autopsy. Many methods to estimate the postmortem interval have been reported, and are typically associated with internal examination. However, there are issues such as rejection of autopsy by the family and a lack of forensic doctor in internal examination. Therefore, it is necessary to develop new methods, such as autopsy imaging, that can substitute for internal examination. Here, we first evaluated whether gas volume in the body increased with postmortem interval. Time-dependent X-ray CT imaging of euthanized Crl:CD (SD) rats (n = 3) was performed immediately after euthanasia and at seven subsequent time points up to 168 h (7 days) at 24-hour intervals. The data revealed that gas volume in the body increased in a time-dependent manner. Next, we reconstructed 3D images of isolated gas and calculated the gas volume using Amira software. In all cases, the volume of both portal venous gas and intestinal gas increased in a time-dependent manner. The volume of portal venous gas increased exponentially, while the volume of intestinal gas increased in a linearly with time. These data might be suggested that the postmortem gas volume change is one of indicators for estimating the postmortem interval. In addition, it would be possible to estimate more accurate postmortem interval by combining not only gas volume changes at the above two sites but also gas volume changes of the other sites such as heart cavities, kidney parenchyma, or abdominal aorta.

UR - http://www.scopus.com/inward/record.url?scp=85044099324&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85044099324&partnerID=8YFLogxK

U2 - 10.1016/j.legalmed.2017.12.010

DO - 10.1016/j.legalmed.2017.12.010

M3 - Article

C2 - 29571154

AN - SCOPUS:85044099324

VL - 32

SP - 66

EP - 70

JO - Legal Medicine

JF - Legal Medicine

SN - 1344-6223

ER -