Potassium iodide-induced changes in pyruvate dehydrogenase complex from bacillus stearothermophilus

Yoichi Aso, Yasuaki Hiromasa, Yoshikatsu Aikawa, Kohji Meno, Masatsune Ishiguro

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Treatment with KI and its subsequent removal induced disassembly of Bacillus stearothermophilus pyruvate dehydrogenase complex (PDC) and association of disassembly products, respectively. The disassembly yielded neither completely dissociated components nor aggregate, but did yield a few molecular forms smaller than PDC. Depending on the KI concentration, these changes were of three phases: K-1, below 0.6 M; K-2, 1.0-1.5 M; K-3, above 1.8 M. PDC was disassembled in K-1 to C1 comprising pyruvate decarboxylase and lipoate acetyltransferase mainly and C2 comprising the decarboxylase and dihydrolipoamide dehydrogenase. In K-1, the removal of KI resulted in an apparent reconstitution of PDC. The mixing of C1 with an excess of C2 yielded an assembly larger than PDC and restored enzyme activities, but specific activities were different from those of PDC. In K-2 and K-3 phases, complexes smaller than PDC were yielded from disassembly products, and activities except for that of the acetyltransferase were not restored.

Original languageEnglish
Pages (from-to)108-116
Number of pages9
JournalBioscience, Biotechnology and Biochemistry
Volume62
Issue number1
DOIs
Publication statusPublished - Jan 1 1998

Fingerprint

Pyruvate Dehydrogenase Complex
Geobacillus stearothermophilus
Potassium Iodide
Bacilli
Dihydrolipoyllysine-Residue Acetyltransferase
Pyruvate Decarboxylase
Dihydrolipoamide Dehydrogenase
Acetyltransferases
Carboxy-Lyases
Enzyme activity
Enzymes

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

Cite this

Potassium iodide-induced changes in pyruvate dehydrogenase complex from bacillus stearothermophilus. / Aso, Yoichi; Hiromasa, Yasuaki; Aikawa, Yoshikatsu; Meno, Kohji; Ishiguro, Masatsune.

In: Bioscience, Biotechnology and Biochemistry, Vol. 62, No. 1, 01.01.1998, p. 108-116.

Research output: Contribution to journalArticle

Aso, Yoichi ; Hiromasa, Yasuaki ; Aikawa, Yoshikatsu ; Meno, Kohji ; Ishiguro, Masatsune. / Potassium iodide-induced changes in pyruvate dehydrogenase complex from bacillus stearothermophilus. In: Bioscience, Biotechnology and Biochemistry. 1998 ; Vol. 62, No. 1. pp. 108-116.
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