Potent cytotoxic effect of the trans10, cis12 isomer of conjugated linoleic acid on rat hepatoma dRLh-84 cells

Masao Yamasaki, Hitomi Chujo, Yasuko Koga, Ayana Oishi, Tatsuya Rikimaru, Mitsuo Shimada, Keizo Sugimachi, Hirofumi Tachibana, Koji Yamada

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Abstract

We evaluated the cytotoxic effect of conjugated linoleic acid (CLA) on rat hepatoma dRLh-84 cells in vitro. When cells were cultured in the presence of CLA, strong cytotoxic effect on dRLh-84 cells was recognized at 1μM level compared to the control vehicle group, and trans10, cis12-CLA but not cis9, trans11-CLA was shown to be an active isomer for inducing this effect. Increase of the sub-G1 population and activation of caspase-3 and 9 accompanied with a time-dependent cleavage of poly(ADP-ribose) polymerase were recognized in dRLh-84 cells treated with trans10, cis12-CLA. In addition, we could see nuclear fragmentation in dRLh-84 cells treated with trans10, cis12-CLA by laser scanning confocal microscopy observation. Cytotoxic effect of trans10, cis12-CLA on normal hepatocytes was weaker than on dRLh-84 cells. These data indicate trans10, cis12-CLA has a potent cytotoxic effect on dRLh-84 cells through at least in part by an apoptotic pathway.

Original languageEnglish
Pages (from-to)171-180
Number of pages10
JournalCancer Letters
Volume188
Issue number1-2
DOIs
Publication statusPublished - Dec 15 2002

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Conjugated Linoleic Acids
Hepatocellular Carcinoma
Poly(ADP-ribose) Polymerases
Caspase 9
Confocal Microscopy
Caspase 3
Hepatocytes
Cultured Cells
Observation
Control Groups

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

Cite this

Yamasaki, M., Chujo, H., Koga, Y., Oishi, A., Rikimaru, T., Shimada, M., ... Yamada, K. (2002). Potent cytotoxic effect of the trans10, cis12 isomer of conjugated linoleic acid on rat hepatoma dRLh-84 cells. Cancer Letters, 188(1-2), 171-180. https://doi.org/10.1016/S0304-3835(02)00459-7

Potent cytotoxic effect of the trans10, cis12 isomer of conjugated linoleic acid on rat hepatoma dRLh-84 cells. / Yamasaki, Masao; Chujo, Hitomi; Koga, Yasuko; Oishi, Ayana; Rikimaru, Tatsuya; Shimada, Mitsuo; Sugimachi, Keizo; Tachibana, Hirofumi; Yamada, Koji.

In: Cancer Letters, Vol. 188, No. 1-2, 15.12.2002, p. 171-180.

Research output: Contribution to journalArticle

Yamasaki, M, Chujo, H, Koga, Y, Oishi, A, Rikimaru, T, Shimada, M, Sugimachi, K, Tachibana, H & Yamada, K 2002, 'Potent cytotoxic effect of the trans10, cis12 isomer of conjugated linoleic acid on rat hepatoma dRLh-84 cells', Cancer Letters, vol. 188, no. 1-2, pp. 171-180. https://doi.org/10.1016/S0304-3835(02)00459-7
Yamasaki, Masao ; Chujo, Hitomi ; Koga, Yasuko ; Oishi, Ayana ; Rikimaru, Tatsuya ; Shimada, Mitsuo ; Sugimachi, Keizo ; Tachibana, Hirofumi ; Yamada, Koji. / Potent cytotoxic effect of the trans10, cis12 isomer of conjugated linoleic acid on rat hepatoma dRLh-84 cells. In: Cancer Letters. 2002 ; Vol. 188, No. 1-2. pp. 171-180.
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