Potentiometric butyrylcholine sensor for organophosphate pesticides

Toshihiko Imato, Nobuhiko Ishibashi

Research output: Contribution to journalArticle

64 Citations (Scopus)

Abstract

A highly sensitive potentiometric butyrylcholine (BuCh) sensor based on plasticized poly(vinyl chloride) membrane was fabricated using tetrakis (3,5-bis[2-methoxy-hexafluoro-methyl] phenyl) borate (HFPB) as a cation exchanger. The sensor showed a Nernstian response from 10-1 M to 10-6 M for BuCh. The detection limit of the BuCh-sensor were improved in comparison with corresponding parameters of BuCh-sensors using prepared other cation exchangers. This improvement was proved to be due to higher hydrophobicity of the cation exchanger, HFPB. Enzyme activity of butyrylcholinesterase (BuChE) was determined by the BuCh-sensor fabricated. Since the selectivity coefficient of the BuCh-sensor for BuCh against a product of the enzyme reaction, choline, was as low as 6·3 × 10-3, the sensor was not affected by interference from choline even when the enzyme reaction proceeded up to about 80%. Michaelis parameters for the BuChE reaction were obtained by the sensor. Determinations of organophosphate pesticides, 2,2-dichlorovinyldimethylphosphate and o-(4-bromo-2-chlorophenyl)o-ethyl S-propylphosphothiolate) were conducted by measuring inhibition of enzyme activity. The enzyme reaction rate was related to the concentration of pesticides. Pesticides were successfully determined between micromole and sub-nanomole levels by the BuCh-sensor.

Original languageEnglish
Pages (from-to)435-441
Number of pages7
JournalBiosensors and Bioelectronics
Volume10
Issue number5
DOIs
Publication statusPublished - Jan 1 1995

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Potentiometric sensors
Organophosphates
Pesticides
Sensors
Ion exchangers
Butyrylcholinesterase
Enzymes
Cations
Borates
Positive ions
Enzyme activity
Choline
Vinyl Chloride
Enzyme inhibition
butyrylcholine
Hydrophobicity
Hydrophobic and Hydrophilic Interactions
Reaction rates
Limit of Detection
Membranes

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

Cite this

Potentiometric butyrylcholine sensor for organophosphate pesticides. / Imato, Toshihiko; Ishibashi, Nobuhiko.

In: Biosensors and Bioelectronics, Vol. 10, No. 5, 01.01.1995, p. 435-441.

Research output: Contribution to journalArticle

Imato, Toshihiko ; Ishibashi, Nobuhiko. / Potentiometric butyrylcholine sensor for organophosphate pesticides. In: Biosensors and Bioelectronics. 1995 ; Vol. 10, No. 5. pp. 435-441.
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