Potentiometric FIA of disaccharides using the hexacyanoferrate(III)-hexacyanoferrate(II) potential buffer solution

Hiroki Ohura, Sumio Yamasaki, Toshihiko Imato, Nobuhiko Ishibashi, Yasukazu Asano

Research output: Contribution to journalArticle

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Abstract

The highly sensitive flow analysis of disaccharides (maltose, lactose, cellobiose and melibiose) was proposed by using a potential buffer solution and an oxidation-reduction potential(ORP) electrode. The analysis was based on the detection of the change in a composition of the buffer solution with the ORP electrode in a redox-reaction of the disaccharide with a hexacyanoferrate(III) ([Fe(CN)6]3−)-hexacyanoferrate(II) ([Fe-(CN)6]4−) potential buffer solution (containing sodium hydroxide). The sample(140 µl) injected into a water stream was merged with the stream of the buffer solution ([Fe(CN)6]3−-[Fc(CN)6]4−; 5 × 10−5–1 × 10−2 mol dm−3) containing sodium hydroxide(0.6 mol dm−3). The reaction was complete for 4.5 min when the sample flows through the reaction tube(0.5 mm i.d., 20 m long) kept at 85°C. The potential change of the ORP electrode was detected as a peak-shaped, and a linear relationship between the peak height and the concentration of disaccharides was observed in the wide range of 1 × 10−6–1.5 × 10−3 mol dm−3. The lower detection limit was 2.5 × 10−7mol dm−3. Sampling rate for determination was about 60 per hour. The relative standard deviation was 1.7–2.9% for analysis of 5 × 10−6 mol dm−3 maltose at sampling rates of 20–60 h−1.

Original languageEnglish
Pages (from-to)807-813
Number of pages7
Journalbunseki kagaku
Volume35
Issue number9
DOIs
Publication statusPublished - Jan 1 1986

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Disaccharides
Buffers
Sodium Hydroxide
Maltose
Electrodes
Melibiose
Sampling
Cellobiose
Redox reactions
Lactose
hexacyanoferrate III
ferrocyn
Water
Chemical analysis
Oxidation-Reduction

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry

Cite this

Potentiometric FIA of disaccharides using the hexacyanoferrate(III)-hexacyanoferrate(II) potential buffer solution. / Ohura, Hiroki; Yamasaki, Sumio; Imato, Toshihiko; Ishibashi, Nobuhiko; Asano, Yasukazu.

In: bunseki kagaku, Vol. 35, No. 9, 01.01.1986, p. 807-813.

Research output: Contribution to journalArticle

Ohura, Hiroki ; Yamasaki, Sumio ; Imato, Toshihiko ; Ishibashi, Nobuhiko ; Asano, Yasukazu. / Potentiometric FIA of disaccharides using the hexacyanoferrate(III)-hexacyanoferrate(II) potential buffer solution. In: bunseki kagaku. 1986 ; Vol. 35, No. 9. pp. 807-813.
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