Practical and functional culture technologies for primary hepatocytes

Research output: Contribution to journalReview article

14 Citations (Scopus)

Abstract

The liver is a central organ for metabolism in the body, and it is therefore important to develop effective culture methods for the expression of liver-specific functions. The development of effective primary hepatocyte culture methods for the expression of these functions has been attempted by focusing on cell-cell and cell-matrix interactions. Two-dimensional organoid culture methods using substrata with the immobilized arginine-glycine-aspartic acid sequence, a well-known cell-adhesive sequence, have been developed. Furthermore, a drug metabolism simulator and a hybrid-type blood purification system as applications of this type of two-dimensional organoid culture method have been developed. On the other hand, growth factor/heparin-immobilized culture substrata and cell-embedded hydrogels in extracellular matrix-filled biocompatible macroporous scaffold culture systems for liver tissue engineering have been developed. Promotion of organoid formation and high expression of the liver-specific functions of primary rat hepatocytes will be realized by using these substrata and culture systems.

Original languageEnglish
Pages (from-to)332-336
Number of pages5
JournalBiochemical Engineering Journal
Volume48
Issue number3
DOIs
Publication statusPublished - Feb 15 2010

Fingerprint

Organoids
Liver
Hepatocytes
Technology
Metabolism
Arginine
Hydrogels
Tissue Engineering
Scaffolds (biology)
Tissue engineering
Aspartic Acid
Cell Communication
Adhesives
Glycine
Purification
Extracellular Matrix
Heparin
Amino acids
Rats
Intercellular Signaling Peptides and Proteins

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Environmental Engineering
  • Bioengineering
  • Biomedical Engineering

Cite this

Practical and functional culture technologies for primary hepatocytes. / Ijima, Hiroyuki.

In: Biochemical Engineering Journal, Vol. 48, No. 3, 15.02.2010, p. 332-336.

Research output: Contribution to journalReview article

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