Practical non-targeted gas chromatography/mass spectrometry-based metabolomics platform for metabolic phenotype analysis

Hiroshi Tsugawa, Takeshi Bamba, Masakazu Shinohara, Shin Nishiumi, Masaru Yoshida, Eiichiro Fukusaki

Research output: Contribution to journalArticlepeer-review

83 Citations (Scopus)

Abstract

Gas chromatography coupled to mass spectrometry (GC/MS) is a core analytical method for metabolomics and has been used as a platform in non-targeted analysis, especially for hydrophilic metabolites. Non-targeted GC/MS-based metabolomics generally requires a high-throughput technology to handle a large volume of samples and an accumulated database (reference library) of the retention times and mass spectra of standard compounds for accurate peak identification. In this study, we provide a practical GC/MS platform and an auto peak identification technique that is not restricted to certain types of mass spectrometers. The platform utilizes a quadrupole mass spectrometer capable of high-speed scanning, resulting in greater output compared with Pegasus GC-time of flight (TOF)/MS, which has been an essential instrument for high-throughput experiments. Moreover, we show that our reference library is broadly applicable to other instruments; peak identification can be readily performed using the library without constructing a reference resource. The usefulness and versatility of our system are demonstrated by the analyses of three experimental metabolomics data sets, including standard mixtures and real biological samples.

Original languageEnglish
Pages (from-to)292-298
Number of pages7
JournalJournal of Bioscience and Bioengineering
Volume112
Issue number3
DOIs
Publication statusPublished - Sep 1 2011
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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