Practical whole-tooth restoration utilizing autologous bioengineered tooth germ transplantation in a postnatal canine model

Mitsuaki Ono, Masamitsu Oshima, Miho Ogawa, Wataru Sonoyama, Emilio Satoshi Hara, Yasutaka Oida, Shigehiko Shinkawa, Ryu Nakajima, Atsushi Mine, Satoru Hayano, Satoshi Fukumoto, Shohei Kasugai, Akira Yamaguchi, Takashi Tsuji, Takuo Kuboki

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Whole-organ regeneration has great potential for the replacement of dysfunctional organs through the reconstruction of a fully functional bioengineered organ using three-dimensional cell manipulation in vitro. Recently, many basic studies of whole-tooth replacement using three-dimensional cell manipulation have been conducted in a mouse model. Further evidence of the practical application to human medicine is required to demonstrate tooth restoration by reconstructing bioengineered tooth germ using a postnatal large-animal model. Herein, we demonstrate functional tooth restoration through the autologous transplantation of bioengineered tooth germ in a postnatal canine model. The bioengineered tooth, which was reconstructed using permanent tooth germ cells, erupted into the jawbone after autologous transplantation and achieved physiological function equivalent to that of a natural tooth. This study represents a substantial advancement in whole-organ replacement therapy through the transplantation of bioengineered organ germ as a practical model for future clinical regenerative medicine.

Original languageEnglish
Article number44522
JournalScientific reports
Volume7
DOIs
Publication statusPublished - Mar 16 2017

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Tooth Germ
Canidae
Tooth
Transplantation
Autologous Transplantation
Regenerative Medicine
Clinical Medicine
Organ Transplantation
Germ Cells
Regeneration
Animal Models
Medicine

All Science Journal Classification (ASJC) codes

  • General

Cite this

Practical whole-tooth restoration utilizing autologous bioengineered tooth germ transplantation in a postnatal canine model. / Ono, Mitsuaki; Oshima, Masamitsu; Ogawa, Miho; Sonoyama, Wataru; Satoshi Hara, Emilio; Oida, Yasutaka; Shinkawa, Shigehiko; Nakajima, Ryu; Mine, Atsushi; Hayano, Satoru; Fukumoto, Satoshi; Kasugai, Shohei; Yamaguchi, Akira; Tsuji, Takashi; Kuboki, Takuo.

In: Scientific reports, Vol. 7, 44522, 16.03.2017.

Research output: Contribution to journalArticle

Ono, M, Oshima, M, Ogawa, M, Sonoyama, W, Satoshi Hara, E, Oida, Y, Shinkawa, S, Nakajima, R, Mine, A, Hayano, S, Fukumoto, S, Kasugai, S, Yamaguchi, A, Tsuji, T & Kuboki, T 2017, 'Practical whole-tooth restoration utilizing autologous bioengineered tooth germ transplantation in a postnatal canine model', Scientific reports, vol. 7, 44522. https://doi.org/10.1038/srep44522
Ono, Mitsuaki ; Oshima, Masamitsu ; Ogawa, Miho ; Sonoyama, Wataru ; Satoshi Hara, Emilio ; Oida, Yasutaka ; Shinkawa, Shigehiko ; Nakajima, Ryu ; Mine, Atsushi ; Hayano, Satoru ; Fukumoto, Satoshi ; Kasugai, Shohei ; Yamaguchi, Akira ; Tsuji, Takashi ; Kuboki, Takuo. / Practical whole-tooth restoration utilizing autologous bioengineered tooth germ transplantation in a postnatal canine model. In: Scientific reports. 2017 ; Vol. 7.
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