Precise comparison of protein localization among OCT, OAT, and MATE in human kidney

Hideyuki Motohashi, Yumi Nakao, Satohiro Masuda, Toshiya Katsura, Tomomi Kamba, Osamu Ogawa, Ken Ichi Inui

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Organic anion transporters (OATs) and organic cation transporters (OCT) play pivotal roles in the uptake of drugs into epithelial cells at the basolateral membranes, and multidrug and toxin extrusion (MATE) mediates drug secretion into urine at the brush-border membranes. In this study, the expression and distribution of apical MATE1 and MATE2-K, and basolateral OAT1, OAT3, and OCT2 were compared using serial sections of human kidney cortex. First, mRNA expression in the proximal tubules was evaluated using laser microdissection. Levels of OAT, OCT2, and MATE mRNA in the proximal tubules were greatly higher compared with glomerulus. The results quantitatively indicated that these transporters were localized to proximal tubules in the renal cortex. Second, MATE1 and MATE2-K protein were detected in proximal epithelial cells in which OCT2 protein was expressed at the basolateral membranes. In addition, MATE1 was expressed at the brush-border membranes of tubular epithelial cells in which OAT1 and OAT3 were expressed. The results confirmed that OAT1, OAT3, OCT2, MATE1, and MATE2-K were coexpressed in tubular epithelial cells. The cooperation among OAT, OCT, and MATE in renal drug secretion was consistent with their distribution.

Original languageEnglish
Pages (from-to)3302-3308
Number of pages7
JournalJournal of Pharmaceutical Sciences
Volume102
Issue number9
DOIs
Publication statusPublished - Jan 1 2013
Externally publishedYes

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Organic Anion Transporters
Cations
Epithelial Cells
Kidney
Membranes
Microvilli
Proteins
Pharmaceutical Preparations
Kidney Cortex
Microdissection
Proximal Kidney Tubule
Messenger RNA
Lasers
Urine

All Science Journal Classification (ASJC) codes

  • Pharmaceutical Science

Cite this

Precise comparison of protein localization among OCT, OAT, and MATE in human kidney. / Motohashi, Hideyuki; Nakao, Yumi; Masuda, Satohiro; Katsura, Toshiya; Kamba, Tomomi; Ogawa, Osamu; Inui, Ken Ichi.

In: Journal of Pharmaceutical Sciences, Vol. 102, No. 9, 01.01.2013, p. 3302-3308.

Research output: Contribution to journalArticle

Motohashi, H, Nakao, Y, Masuda, S, Katsura, T, Kamba, T, Ogawa, O & Inui, KI 2013, 'Precise comparison of protein localization among OCT, OAT, and MATE in human kidney', Journal of Pharmaceutical Sciences, vol. 102, no. 9, pp. 3302-3308. https://doi.org/10.1002/jps.23567
Motohashi, Hideyuki ; Nakao, Yumi ; Masuda, Satohiro ; Katsura, Toshiya ; Kamba, Tomomi ; Ogawa, Osamu ; Inui, Ken Ichi. / Precise comparison of protein localization among OCT, OAT, and MATE in human kidney. In: Journal of Pharmaceutical Sciences. 2013 ; Vol. 102, No. 9. pp. 3302-3308.
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