Prediction of periodontal inflammation via metabolic profiling of saliva

M. Kuboniwa, A. Sakanaka, E. Hashino, T. Bamba, E. Fukusaki, A. Amano

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Periodontal disease is characterized by chronic inflammation in subgingival areas, where a vast array of inflammation-associated metabolites are likely produced from tissue breakdown, increased vascular permeability, and microbial metabolism and then eventually show a steady flow into saliva. Thus, prolonged periodontal inflammation is a key feature of disease activity. Although salivary metabolomics has drawn attention for its potential use in diagnosis of periodontal disease, few authors have used that to investigate periodontal inflammation detection. In this pilot study, the authors explored the use of salivary metabolites to reflect periodontal inflammation severity with a recently proposed parameter - periodontal inflamed surface area (PISA) - used to quantify the periodontal inflammatory burden of individual patients with high accuracy. Following PISA determination, whole saliva samples were collected from 19 subjects before and after removal of supragingival plaque and calculus (debridement) with an ultrasonic scaler to assess the influence of the procedure on salivary metabolic profiles. Metabolic profiling of saliva was performed with gas chromatography coupled to time-of-flight mass spectrometry, followed by multivariate regression analysis with orthogonal projections to latent structures (OPLS) to investigate the relationship between PISA and salivary metabolic profiles. Sixty-three metabolites were identified. OPLS analysis showed that postdebridement saliva provided a more refined model for prediction of PISA than did predebridement samples, which indicated that debridement may improve detection of metabolites eluted from subgingival areas in saliva, thus more accurately reflecting the pathophysiology of periodontitis. Based on the variable importance in the projection values obtained via OPLS, 8 metabolites were identified as potential indicators of periodontal inflammation, of which the combination of cadaverine, 5-oxoproline, and histidine yielded satisfactory accuracy (area under the curve = 0.881) for diagnosis of periodontitis. The authors' findings identified potential biomarkers that may be useful for reflecting the severity of periodontal inflammation as part of monitoring disease activity in periodontitis patients.

Original languageEnglish
Pages (from-to)1381-1386
Number of pages6
JournalJournal of Dental Research
Volume95
Issue number12
DOIs
Publication statusPublished - Nov 1 2016

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Saliva
Inflammation
Periodontitis
Metabolome
Periodontal Diseases
Debridement
Pyrrolidonecarboxylic Acid
Cadaverine
Metabolomics
Calculi
Capillary Permeability
Histidine
Ultrasonics
Gas Chromatography
Area Under Curve
Mass Spectrometry
Multivariate Analysis
Biomarkers
Regression Analysis

All Science Journal Classification (ASJC) codes

  • Dentistry(all)

Cite this

Prediction of periodontal inflammation via metabolic profiling of saliva. / Kuboniwa, M.; Sakanaka, A.; Hashino, E.; Bamba, T.; Fukusaki, E.; Amano, A.

In: Journal of Dental Research, Vol. 95, No. 12, 01.11.2016, p. 1381-1386.

Research output: Contribution to journalArticle

Kuboniwa, M, Sakanaka, A, Hashino, E, Bamba, T, Fukusaki, E & Amano, A 2016, 'Prediction of periodontal inflammation via metabolic profiling of saliva', Journal of Dental Research, vol. 95, no. 12, pp. 1381-1386. https://doi.org/10.1177/0022034516661142
Kuboniwa, M. ; Sakanaka, A. ; Hashino, E. ; Bamba, T. ; Fukusaki, E. ; Amano, A. / Prediction of periodontal inflammation via metabolic profiling of saliva. In: Journal of Dental Research. 2016 ; Vol. 95, No. 12. pp. 1381-1386.
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