Abstract
γδ T cell receptor-positive cells (γδ T cells) have recently been implicated to play a role in the protection against infectious pathogens. Serial studies on γδ T cells in 14 patients with salmonella infection have revealed that the proportions of γδ T cells (mean±SD: 17.9±13.2%) in salmonella infection were significantly increased (P < 0.01) compared with 35 normal controls (5.0±2.6%) and 13 patients with other bacterial infections (4.0±1.4%). Expansion of γδ T cells was more prominent in the systemic form (28.9±10.8%) than in the gastroenteritis form (10.5±7.9%) of salmonella infection (P < 0.01). Most in vivo-expanded γδ T cells expressed Vγ 9 gene product. Increased activated (HLA-DR+) T cells were observed in all the six patients with the systemic form and four of the seven with gastroenteritis form. Especially in the six with systemic form, γδ T cell activation was significantly higher than αβ T cell activation at the early stage of illness (P < 0.01). When peripheral blood lymphocytes from normal individuals were cultured with live salmonella, γδ T cells were preferentially activated and expanded and most of them expressed Vγ 9. Purified γδ T cells also responded to live salmonella in vitro. The present study suggests that human γδ T cells play a role in the protection against salmonella infection in vivo.
| Original language | English |
|---|---|
| Pages (from-to) | 204-210 |
| Number of pages | 7 |
| Journal | Journal of Clinical Investigation |
| Volume | 90 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Jan 1 1992 |
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All Science Journal Classification (ASJC) codes
- Medicine(all)
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Predominant activation and expansion of Vγ9-bearing γδT cells in vivo as well as in vitro in salmonella infection. / Hara, T.; Mizuno, Y.; Takaki, K.; Takada, H.; Akeda, H.; Aoki, T.; Nagata, M.; Ueda, K.; Matsuzaki, G.; Yoshikai, Y.; Nomoto, K.
In: Journal of Clinical Investigation, Vol. 90, No. 1, 01.01.1992, p. 204-210.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Predominant activation and expansion of Vγ9-bearing γδT cells in vivo as well as in vitro in salmonella infection
AU - Hara, T.
AU - Mizuno, Y.
AU - Takaki, K.
AU - Takada, H.
AU - Akeda, H.
AU - Aoki, T.
AU - Nagata, M.
AU - Ueda, K.
AU - Matsuzaki, G.
AU - Yoshikai, Y.
AU - Nomoto, K.
PY - 1992/1/1
Y1 - 1992/1/1
N2 - γδ T cell receptor-positive cells (γδ T cells) have recently been implicated to play a role in the protection against infectious pathogens. Serial studies on γδ T cells in 14 patients with salmonella infection have revealed that the proportions of γδ T cells (mean±SD: 17.9±13.2%) in salmonella infection were significantly increased (P < 0.01) compared with 35 normal controls (5.0±2.6%) and 13 patients with other bacterial infections (4.0±1.4%). Expansion of γδ T cells was more prominent in the systemic form (28.9±10.8%) than in the gastroenteritis form (10.5±7.9%) of salmonella infection (P < 0.01). Most in vivo-expanded γδ T cells expressed Vγ 9 gene product. Increased activated (HLA-DR+) T cells were observed in all the six patients with the systemic form and four of the seven with gastroenteritis form. Especially in the six with systemic form, γδ T cell activation was significantly higher than αβ T cell activation at the early stage of illness (P < 0.01). When peripheral blood lymphocytes from normal individuals were cultured with live salmonella, γδ T cells were preferentially activated and expanded and most of them expressed Vγ 9. Purified γδ T cells also responded to live salmonella in vitro. The present study suggests that human γδ T cells play a role in the protection against salmonella infection in vivo.
AB - γδ T cell receptor-positive cells (γδ T cells) have recently been implicated to play a role in the protection against infectious pathogens. Serial studies on γδ T cells in 14 patients with salmonella infection have revealed that the proportions of γδ T cells (mean±SD: 17.9±13.2%) in salmonella infection were significantly increased (P < 0.01) compared with 35 normal controls (5.0±2.6%) and 13 patients with other bacterial infections (4.0±1.4%). Expansion of γδ T cells was more prominent in the systemic form (28.9±10.8%) than in the gastroenteritis form (10.5±7.9%) of salmonella infection (P < 0.01). Most in vivo-expanded γδ T cells expressed Vγ 9 gene product. Increased activated (HLA-DR+) T cells were observed in all the six patients with the systemic form and four of the seven with gastroenteritis form. Especially in the six with systemic form, γδ T cell activation was significantly higher than αβ T cell activation at the early stage of illness (P < 0.01). When peripheral blood lymphocytes from normal individuals were cultured with live salmonella, γδ T cells were preferentially activated and expanded and most of them expressed Vγ 9. Purified γδ T cells also responded to live salmonella in vitro. The present study suggests that human γδ T cells play a role in the protection against salmonella infection in vivo.
UR - http://www.scopus.com/inward/record.url?scp=0026696328&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0026696328&partnerID=8YFLogxK
U2 - 10.1172/JCI115837
DO - 10.1172/JCI115837
M3 - Article
C2 - 1386086
AN - SCOPUS:0026696328
VL - 90
SP - 204
EP - 210
JO - Journal of Clinical Investigation
JF - Journal of Clinical Investigation
SN - 0021-9738
IS - 1
ER -