Predominant activation and expansion of Vγ9-bearing γδT cells in vivo as well as in vitro in salmonella infection

T. Hara, Y. Mizuno, K. Takaki, H. Takada, H. Akeda, T. Aoki, M. Nagata, K. Ueda, G. Matsuzaki, Y. Yoshikai, K. Nomoto

Research output: Contribution to journalArticle

132 Citations (Scopus)

Abstract

γδ T cell receptor-positive cells (γδ T cells) have recently been implicated to play a role in the protection against infectious pathogens. Serial studies on γδ T cells in 14 patients with salmonella infection have revealed that the proportions of γδ T cells (mean±SD: 17.9±13.2%) in salmonella infection were significantly increased (P < 0.01) compared with 35 normal controls (5.0±2.6%) and 13 patients with other bacterial infections (4.0±1.4%). Expansion of γδ T cells was more prominent in the systemic form (28.9±10.8%) than in the gastroenteritis form (10.5±7.9%) of salmonella infection (P < 0.01). Most in vivo-expanded γδ T cells expressed Vγ 9 gene product. Increased activated (HLA-DR+) T cells were observed in all the six patients with the systemic form and four of the seven with gastroenteritis form. Especially in the six with systemic form, γδ T cell activation was significantly higher than αβ T cell activation at the early stage of illness (P < 0.01). When peripheral blood lymphocytes from normal individuals were cultured with live salmonella, γδ T cells were preferentially activated and expanded and most of them expressed Vγ 9. Purified γδ T cells also responded to live salmonella in vitro. The present study suggests that human γδ T cells play a role in the protection against salmonella infection in vivo.

Original languageEnglish
Pages (from-to)204-210
Number of pages7
JournalJournal of Clinical Investigation
Volume90
Issue number1
DOIs
Publication statusPublished - Jan 1 1992

Fingerprint

Salmonella Infections
T-Cell Antigen Receptor
Gastroenteritis
Salmonella
In Vitro Techniques
HLA-DR Antigens
Bacterial Infections
Lymphocytes

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

Predominant activation and expansion of Vγ9-bearing γδT cells in vivo as well as in vitro in salmonella infection. / Hara, T.; Mizuno, Y.; Takaki, K.; Takada, H.; Akeda, H.; Aoki, T.; Nagata, M.; Ueda, K.; Matsuzaki, G.; Yoshikai, Y.; Nomoto, K.

In: Journal of Clinical Investigation, Vol. 90, No. 1, 01.01.1992, p. 204-210.

Research output: Contribution to journalArticle

Hara, T, Mizuno, Y, Takaki, K, Takada, H, Akeda, H, Aoki, T, Nagata, M, Ueda, K, Matsuzaki, G, Yoshikai, Y & Nomoto, K 1992, 'Predominant activation and expansion of Vγ9-bearing γδT cells in vivo as well as in vitro in salmonella infection', Journal of Clinical Investigation, vol. 90, no. 1, pp. 204-210. https://doi.org/10.1172/JCI115837
Hara, T. ; Mizuno, Y. ; Takaki, K. ; Takada, H. ; Akeda, H. ; Aoki, T. ; Nagata, M. ; Ueda, K. ; Matsuzaki, G. ; Yoshikai, Y. ; Nomoto, K. / Predominant activation and expansion of Vγ9-bearing γδT cells in vivo as well as in vitro in salmonella infection. In: Journal of Clinical Investigation. 1992 ; Vol. 90, No. 1. pp. 204-210.
@article{b371da9c6f564a039020f20320b14ae4,
title = "Predominant activation and expansion of Vγ9-bearing γδT cells in vivo as well as in vitro in salmonella infection",
abstract = "γδ T cell receptor-positive cells (γδ T cells) have recently been implicated to play a role in the protection against infectious pathogens. Serial studies on γδ T cells in 14 patients with salmonella infection have revealed that the proportions of γδ T cells (mean±SD: 17.9±13.2{\%}) in salmonella infection were significantly increased (P < 0.01) compared with 35 normal controls (5.0±2.6{\%}) and 13 patients with other bacterial infections (4.0±1.4{\%}). Expansion of γδ T cells was more prominent in the systemic form (28.9±10.8{\%}) than in the gastroenteritis form (10.5±7.9{\%}) of salmonella infection (P < 0.01). Most in vivo-expanded γδ T cells expressed Vγ 9 gene product. Increased activated (HLA-DR+) T cells were observed in all the six patients with the systemic form and four of the seven with gastroenteritis form. Especially in the six with systemic form, γδ T cell activation was significantly higher than αβ T cell activation at the early stage of illness (P < 0.01). When peripheral blood lymphocytes from normal individuals were cultured with live salmonella, γδ T cells were preferentially activated and expanded and most of them expressed Vγ 9. Purified γδ T cells also responded to live salmonella in vitro. The present study suggests that human γδ T cells play a role in the protection against salmonella infection in vivo.",
author = "T. Hara and Y. Mizuno and K. Takaki and H. Takada and H. Akeda and T. Aoki and M. Nagata and K. Ueda and G. Matsuzaki and Y. Yoshikai and K. Nomoto",
year = "1992",
month = "1",
day = "1",
doi = "10.1172/JCI115837",
language = "English",
volume = "90",
pages = "204--210",
journal = "Journal of Clinical Investigation",
issn = "0021-9738",
publisher = "The American Society for Clinical Investigation",
number = "1",

}

TY - JOUR

T1 - Predominant activation and expansion of Vγ9-bearing γδT cells in vivo as well as in vitro in salmonella infection

AU - Hara, T.

AU - Mizuno, Y.

AU - Takaki, K.

AU - Takada, H.

AU - Akeda, H.

AU - Aoki, T.

AU - Nagata, M.

AU - Ueda, K.

AU - Matsuzaki, G.

AU - Yoshikai, Y.

AU - Nomoto, K.

PY - 1992/1/1

Y1 - 1992/1/1

N2 - γδ T cell receptor-positive cells (γδ T cells) have recently been implicated to play a role in the protection against infectious pathogens. Serial studies on γδ T cells in 14 patients with salmonella infection have revealed that the proportions of γδ T cells (mean±SD: 17.9±13.2%) in salmonella infection were significantly increased (P < 0.01) compared with 35 normal controls (5.0±2.6%) and 13 patients with other bacterial infections (4.0±1.4%). Expansion of γδ T cells was more prominent in the systemic form (28.9±10.8%) than in the gastroenteritis form (10.5±7.9%) of salmonella infection (P < 0.01). Most in vivo-expanded γδ T cells expressed Vγ 9 gene product. Increased activated (HLA-DR+) T cells were observed in all the six patients with the systemic form and four of the seven with gastroenteritis form. Especially in the six with systemic form, γδ T cell activation was significantly higher than αβ T cell activation at the early stage of illness (P < 0.01). When peripheral blood lymphocytes from normal individuals were cultured with live salmonella, γδ T cells were preferentially activated and expanded and most of them expressed Vγ 9. Purified γδ T cells also responded to live salmonella in vitro. The present study suggests that human γδ T cells play a role in the protection against salmonella infection in vivo.

AB - γδ T cell receptor-positive cells (γδ T cells) have recently been implicated to play a role in the protection against infectious pathogens. Serial studies on γδ T cells in 14 patients with salmonella infection have revealed that the proportions of γδ T cells (mean±SD: 17.9±13.2%) in salmonella infection were significantly increased (P < 0.01) compared with 35 normal controls (5.0±2.6%) and 13 patients with other bacterial infections (4.0±1.4%). Expansion of γδ T cells was more prominent in the systemic form (28.9±10.8%) than in the gastroenteritis form (10.5±7.9%) of salmonella infection (P < 0.01). Most in vivo-expanded γδ T cells expressed Vγ 9 gene product. Increased activated (HLA-DR+) T cells were observed in all the six patients with the systemic form and four of the seven with gastroenteritis form. Especially in the six with systemic form, γδ T cell activation was significantly higher than αβ T cell activation at the early stage of illness (P < 0.01). When peripheral blood lymphocytes from normal individuals were cultured with live salmonella, γδ T cells were preferentially activated and expanded and most of them expressed Vγ 9. Purified γδ T cells also responded to live salmonella in vitro. The present study suggests that human γδ T cells play a role in the protection against salmonella infection in vivo.

UR - http://www.scopus.com/inward/record.url?scp=0026696328&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026696328&partnerID=8YFLogxK

U2 - 10.1172/JCI115837

DO - 10.1172/JCI115837

M3 - Article

C2 - 1386086

AN - SCOPUS:0026696328

VL - 90

SP - 204

EP - 210

JO - Journal of Clinical Investigation

JF - Journal of Clinical Investigation

SN - 0021-9738

IS - 1

ER -