Predominant activation and expansion of Vγ9-bearing γδT cells in vivo as well as in vitro in salmonella infection

T. Hara, Y. Mizuno, K. Takaki, H. Takada, H. Akeda, T. Aoki, M. Nagata, K. Ueda, G. Matsuzaki, Y. Yoshikai, K. Nomoto

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Abstract

γδ T cell receptor-positive cells (γδ T cells) have recently been implicated to play a role in the protection against infectious pathogens. Serial studies on γδ T cells in 14 patients with salmonella infection have revealed that the proportions of γδ T cells (mean±SD: 17.9±13.2%) in salmonella infection were significantly increased (P < 0.01) compared with 35 normal controls (5.0±2.6%) and 13 patients with other bacterial infections (4.0±1.4%). Expansion of γδ T cells was more prominent in the systemic form (28.9±10.8%) than in the gastroenteritis form (10.5±7.9%) of salmonella infection (P < 0.01). Most in vivo-expanded γδ T cells expressed Vγ 9 gene product. Increased activated (HLA-DR+) T cells were observed in all the six patients with the systemic form and four of the seven with gastroenteritis form. Especially in the six with systemic form, γδ T cell activation was significantly higher than αβ T cell activation at the early stage of illness (P < 0.01). When peripheral blood lymphocytes from normal individuals were cultured with live salmonella, γδ T cells were preferentially activated and expanded and most of them expressed Vγ 9. Purified γδ T cells also responded to live salmonella in vitro. The present study suggests that human γδ T cells play a role in the protection against salmonella infection in vivo.

Original languageEnglish
Pages (from-to)204-210
Number of pages7
JournalJournal of Clinical Investigation
Volume90
Issue number1
DOIs
Publication statusPublished - 1992

All Science Journal Classification (ASJC) codes

  • Medicine(all)

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    Hara, T., Mizuno, Y., Takaki, K., Takada, H., Akeda, H., Aoki, T., Nagata, M., Ueda, K., Matsuzaki, G., Yoshikai, Y., & Nomoto, K. (1992). Predominant activation and expansion of Vγ9-bearing γδT cells in vivo as well as in vitro in salmonella infection. Journal of Clinical Investigation, 90(1), 204-210. https://doi.org/10.1172/JCI115837