Preferential expression of the vasoactive intestinal peptide (VIP) receptor VPAC1 in human cord blood-derived CD34+ CD38- cells: Possible role of VIP as a growth-promoting factor for hematopoietic stem/progenitor cells

M. Kawakami, T. Kimura, Y. Kishimoto, T. Tatekawa, Yoshihiro Baba, T. Nishizaki, N. Matsuzaki, T. Taniguchi, S. Yoshihara, K. Ikegame, T. Shirakata, S. Nishida, T. Masuda, N. Hosen, A. Tsuboi, Y. Oji, Y. Oka, H. Ogawa, Y. Sonoda, H. SugiyamaI. Kawase, T. Soma

Research output: Contribution to journalArticle

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Abstract

Primitive hematopoietic progenitor cells such as severe combined immunodeficiency- repopulating cells and long-term culture-initiating cells are enriched in CD34+ CD38- cells derived from various stem cell sources. In this study, to elucidate the features of such primitive cells at the molecular level, we tried to isolate genes that were preferentially expressed in umbilical cord blood (CB -derived CD34+CD38- cells by subtractive hybridization. The gene for VPAC1 receptor, a receptor for the neuropeptide vasoactive intestinal peptide (VIP), was thereby isolated and it was shown that this gene was expressed in both CD34+CD38- and CD34+CD38+ CB cells and that the expression levels were higher in CD34+CD38 CB cells. Next, we assessed the effects of VIP on the proliferation of CD34+ CB cells using in vitro culture systems. In serum-free single-cell suspension culture, VIP enhanced clonal growth of CD34+ CB cells in synergy with FLT3 ligand (FL), stem cell factor (SCF), and thrombopoietin (TPO). In serum-free clonogenic assays, VIP promoted myeloid (colony-forming unit-granulocyte/macrophage (CFU-GM) and mixed (CFU-Mix) colony formations. Furthermore, in Dexter-type long-term cultures, VIP increased colony-forming cells at week 5 of culture. These results suggest that VIP functions as a growth-promoting factor of CB-derived hematopoetic progenitor cells.

Original languageEnglish
Pages (from-to)912-921
Number of pages10
JournalLeukemia
Volume18
Issue number5
DOIs
Publication statusPublished - Jan 1 2004
Externally publishedYes

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Vasoactive Intestinal Peptide Receptors
Vasoactive Intestinal Peptide
Hematopoietic Stem Cells
Fetal Blood
Intercellular Signaling Peptides and Proteins
Receptors, Vasoactive Intestinal Polypeptide, Type I
Stem Cells
Cell Culture Techniques
Genes
Thrombopoietin
Severe Combined Immunodeficiency
Granulocyte-Macrophage Progenitor Cells
Stem Cell Factor
Serum
Suspensions
Ligands

All Science Journal Classification (ASJC) codes

  • Hematology
  • Oncology
  • Cancer Research

Cite this

Preferential expression of the vasoactive intestinal peptide (VIP) receptor VPAC1 in human cord blood-derived CD34+ CD38- cells : Possible role of VIP as a growth-promoting factor for hematopoietic stem/progenitor cells. / Kawakami, M.; Kimura, T.; Kishimoto, Y.; Tatekawa, T.; Baba, Yoshihiro; Nishizaki, T.; Matsuzaki, N.; Taniguchi, T.; Yoshihara, S.; Ikegame, K.; Shirakata, T.; Nishida, S.; Masuda, T.; Hosen, N.; Tsuboi, A.; Oji, Y.; Oka, Y.; Ogawa, H.; Sonoda, Y.; Sugiyama, H.; Kawase, I.; Soma, T.

In: Leukemia, Vol. 18, No. 5, 01.01.2004, p. 912-921.

Research output: Contribution to journalArticle

Kawakami, M, Kimura, T, Kishimoto, Y, Tatekawa, T, Baba, Y, Nishizaki, T, Matsuzaki, N, Taniguchi, T, Yoshihara, S, Ikegame, K, Shirakata, T, Nishida, S, Masuda, T, Hosen, N, Tsuboi, A, Oji, Y, Oka, Y, Ogawa, H, Sonoda, Y, Sugiyama, H, Kawase, I & Soma, T 2004, 'Preferential expression of the vasoactive intestinal peptide (VIP) receptor VPAC1 in human cord blood-derived CD34+ CD38- cells: Possible role of VIP as a growth-promoting factor for hematopoietic stem/progenitor cells', Leukemia, vol. 18, no. 5, pp. 912-921. https://doi.org/10.1038/sj.leu.2403330
Kawakami, M. ; Kimura, T. ; Kishimoto, Y. ; Tatekawa, T. ; Baba, Yoshihiro ; Nishizaki, T. ; Matsuzaki, N. ; Taniguchi, T. ; Yoshihara, S. ; Ikegame, K. ; Shirakata, T. ; Nishida, S. ; Masuda, T. ; Hosen, N. ; Tsuboi, A. ; Oji, Y. ; Oka, Y. ; Ogawa, H. ; Sonoda, Y. ; Sugiyama, H. ; Kawase, I. ; Soma, T. / Preferential expression of the vasoactive intestinal peptide (VIP) receptor VPAC1 in human cord blood-derived CD34+ CD38- cells : Possible role of VIP as a growth-promoting factor for hematopoietic stem/progenitor cells. In: Leukemia. 2004 ; Vol. 18, No. 5. pp. 912-921.
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T2 - Possible role of VIP as a growth-promoting factor for hematopoietic stem/progenitor cells

AU - Kawakami, M.

AU - Kimura, T.

AU - Kishimoto, Y.

AU - Tatekawa, T.

AU - Baba, Yoshihiro

AU - Nishizaki, T.

AU - Matsuzaki, N.

AU - Taniguchi, T.

AU - Yoshihara, S.

AU - Ikegame, K.

AU - Shirakata, T.

AU - Nishida, S.

AU - Masuda, T.

AU - Hosen, N.

AU - Tsuboi, A.

AU - Oji, Y.

AU - Oka, Y.

AU - Ogawa, H.

AU - Sonoda, Y.

AU - Sugiyama, H.

AU - Kawase, I.

AU - Soma, T.

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