Pregnenolone stabilizes microtubules and promotes zebrafish embryonic cell movement

Hwei Jan Hsu, Ming Ren Liang, Chao Tsen Chen, Bon Chu Chung

Research output: Contribution to journalArticle

69 Citations (Scopus)

Abstract

Embryonic cell movement is essential for morphogenesis and the establishment of body shapes1,2, but little is known about its mechanism. Here we report that pregnenolone, which is produced from cholesterol by the steroidogenic enzyme Cyp11a1 (cholesterol side-chain cleavage enzyme, P450scc)3, functions in promoting cell migration during epiboly. Epiboly is a process in which embryonic cells spread from the animal pole to cover the underlying yolk. During epiboly, cyp11a1 is expressed in an extra-embryonic yolk syncytial layer4. Reducing cyp11a1 expression in zebrafish using antisense morpholino oligonucleotides did not perturb cell fates, but caused epibolic delay. This epibolic defect was partially rescued by the injection of cyp11a1 RNA or the supplementation of pregnenolone. We show that the epibolic delay is accompanied by a decrease in the level of polymerized microtubules, and that pregnenolone can rescue this microtubule defect. Our results indicate that pregnenolone preserves microtubule abundance and promotes cell movement during epiboly.

Original languageEnglish
Pages (from-to)480-483
Number of pages4
JournalNature
Volume439
Issue number7075
DOIs
Publication statusPublished - Jan 26 2006

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Pregnenolone
Zebrafish
Microtubules
Cell Movement
Cholesterol Side-Chain Cleavage Enzyme
Morpholinos
Antisense Oligonucleotides
Morphogenesis
Cholesterol
RNA
Injections
Enzymes

All Science Journal Classification (ASJC) codes

  • General

Cite this

Hsu, H. J., Liang, M. R., Chen, C. T., & Chung, B. C. (2006). Pregnenolone stabilizes microtubules and promotes zebrafish embryonic cell movement. Nature, 439(7075), 480-483. https://doi.org/10.1038/nature04436

Pregnenolone stabilizes microtubules and promotes zebrafish embryonic cell movement. / Hsu, Hwei Jan; Liang, Ming Ren; Chen, Chao Tsen; Chung, Bon Chu.

In: Nature, Vol. 439, No. 7075, 26.01.2006, p. 480-483.

Research output: Contribution to journalArticle

Hsu, HJ, Liang, MR, Chen, CT & Chung, BC 2006, 'Pregnenolone stabilizes microtubules and promotes zebrafish embryonic cell movement', Nature, vol. 439, no. 7075, pp. 480-483. https://doi.org/10.1038/nature04436
Hsu, Hwei Jan ; Liang, Ming Ren ; Chen, Chao Tsen ; Chung, Bon Chu. / Pregnenolone stabilizes microtubules and promotes zebrafish embryonic cell movement. In: Nature. 2006 ; Vol. 439, No. 7075. pp. 480-483.
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