Preimplantation embryo-secreted factors modulate maternal gene expression in rat uterus

Kazuki Yamagami, M. Rashedul Islam, Yuka Yoshii, Kazuki Mori, Kosuke Tashiro, Nobuhiko Yamauchi

Research output: Contribution to journalArticle

Abstract

In mammalian reproduction, embryo implantation into the uterus is spatiotemporally regulated by a complex process triggered by a number of factors. Although previous studies have suggested that uterine receptivity is mediated by blastocyst-derived factors, specific functions of embryos remain to be defined during preimplantation. Therefore, the present study was conducted to identify the maternal genes regulated by embryo-secreted factors in the rat uterus. RNA-sequencing (RNA-seq) data revealed that 10 genes are up-regulated in the delayed implantation uterus compared with the pseudopregnancy uterus. The RNA-seq results were further verified by real-time quantitative polymerase chain reaction. Sulf1 expression is significantly (P < 0.05) induced in the delayed implantation uterus, although Areg, Calca, Fxyd4 and Lamc3 show a definite but non-statistically significant increase in their expression levels. During early pregnancy, the levels of Areg, Calca, Fxyd4, Lamc3 and Sulf1 expression at 3.5 days post coitus (dpc) are significantly (P < 0.05) higher than those at 1.5 dpc. Treatment with embryo-conditioned media revealed that Lamc3 and Sulf1 are up-regulated compared with the other genes studied. Thus, embryo-derived factors regulate maternal gene expression, with Lamc3 and Sulf1 possibly being suitable markers for a response study of embryo-secreted factors to improve our understanding of embryo-maternal communication.

Original languageEnglish
Pages (from-to)453-463
Number of pages11
JournalCell and tissue research
Volume364
Issue number2
DOIs
Publication statusPublished - May 1 2016

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Blastocyst
Uterus
Embryonic Structures
Mothers
Gene Expression
RNA
Pseudopregnancy
Genes
RNA Sequence Analysis
Coitus
Conditioned Culture Medium
Reproduction
Real-Time Polymerase Chain Reaction
Communication
Pregnancy

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine
  • Histology
  • Cell Biology

Cite this

Preimplantation embryo-secreted factors modulate maternal gene expression in rat uterus. / Yamagami, Kazuki; Islam, M. Rashedul; Yoshii, Yuka; Mori, Kazuki; Tashiro, Kosuke; Yamauchi, Nobuhiko.

In: Cell and tissue research, Vol. 364, No. 2, 01.05.2016, p. 453-463.

Research output: Contribution to journalArticle

Yamagami, Kazuki ; Islam, M. Rashedul ; Yoshii, Yuka ; Mori, Kazuki ; Tashiro, Kosuke ; Yamauchi, Nobuhiko. / Preimplantation embryo-secreted factors modulate maternal gene expression in rat uterus. In: Cell and tissue research. 2016 ; Vol. 364, No. 2. pp. 453-463.
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