Preliminary study on in situ realtime quantitation of target bacteria on the principle of flow cytometry

Gen Murakami, Yuichi Sugai, Kyuro Sasaki

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

In-situ realtime method that can monitor the target bacteria should be used to determine the real situation of the bacteria in deep parts of heaps in heap bioleaching plants. This study suggest to apply flow cytometry technology to in-situ realtime monitoring of target bacteria. Flow cytometry is a method that can rapidly quantify the bacterial cells in bacterial suspension based on the detection of lights that are emitted from bacterial cells. In this study, we estimated the possibility of the application of flow cytometry to the selective detection of target bacteria. The bacterial culture solution that had been diluted by water including other bacteria was provided for fluorescence spectral analysis and scattered light analysis that were functions of flow cytometry. Our target bacteria could be selectively detected by those analyses in this study, therefore, it was shown that the flow cytometry could be useful for detecting target bacteria selectively. Because the measurement principle of flow cytometry is quite simple, it can be expected to be installed into deep heaps through the monitoring wells and determine the dominance of target bacteria in-situ and realtime in the future.

Original languageEnglish
Title of host publication22nd International Biohydrometallurgy Symposium
EditorsSabrina Hedrich, Axel Schippers, Kathrin Rubberdt, Franz Glombitza, Wolfgang Sand, Wolfgang Sand, Mario Vera Veliz, Sabine Willscher
Place of Publication Switzerland
PublisherTrans Tech Publications Ltd
Pages224-227
Number of pages4
Volume262
ISBN (Print)9783035711806
DOIs
Publication statusPublished - Aug 2017
Event22nd International Biohydrometallurgy Symposium, 2017 - Freiberg, Germany
Duration: Sep 24 2017Sep 27 2017

Publication series

NameSolid State Phenomena
Volume262 SSP
ISSN (Print)1012-0394
ISSN (Electronic)1662-9779

Other

Other22nd International Biohydrometallurgy Symposium, 2017
CountryGermany
CityFreiberg
Period9/24/179/27/17

All Science Journal Classification (ASJC) codes

  • Fuel Technology
  • Biochemistry

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    Murakami, G., Sugai, Y., & Sasaki, K. (2017). Preliminary study on in situ realtime quantitation of target bacteria on the principle of flow cytometry. In S. Hedrich, A. Schippers, K. Rubberdt, F. Glombitza, W. Sand, W. Sand, M. V. Veliz, & S. Willscher (Eds.), 22nd International Biohydrometallurgy Symposium (Vol. 262, pp. 224-227). (Solid State Phenomena; Vol. 262 SSP). Trans Tech Publications Ltd. https://doi.org/10.4028/www.scientific.net/SSP.262.224