Preliminary study on the receptor of gonad-stimulating substance (GSS) as a gonadotropin of starfish

Masatoshi Mita, Kazutoshi Yamamoto, Michiyasu Yoshikuni, Kaoru Ohno, Yoshitaka Nagahama

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Previously, the gonad-stimulating substance (GSS) which acts as the gonadotropin was purified from the starfish (Asterina pectinifera) and subsequently, its structure was deciphered. In this study, artificial GSS was synthesized and its interaction with the receptors was examined further. According to competitive experiments using radioiodinated and radioinert GSS in various tissues of A. pectinifera, high specific bindings were observed in the ovarian follicle cells and testicular interstitial cells. Scatchard plot analysis also showed that Kd values were about 4 nM in follicle cells and about 7 nM in interstitial cells. The numbers of binding sites in follicle cells were estimated to be about 3 pmoles/mg protein and in interstitial cells to be about 1 pmoles/mg protein. These strongly suggest that GSS receptors are distributed to follicle cells in female and interstitial cells in male, respectively.

Original languageEnglish
Pages (from-to)299-301
Number of pages3
JournalGeneral and Comparative Endocrinology
Volume153
Issue number1-3
DOIs
Publication statusPublished - Jan 1 2007
Externally publishedYes

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Starfish
Asteroidea
gonadotropins
Gonadotropins
gonads
receptors
Asterina
Gonads
Asterina pectinifera
cells
Leydig cells
Ovarian Follicle
Leydig Cells
ovarian follicles
gonad-stimulatingsubstance receptor
binding sites
Proteins
proteins
Binding Sites

All Science Journal Classification (ASJC) codes

  • Endocrinology

Cite this

Preliminary study on the receptor of gonad-stimulating substance (GSS) as a gonadotropin of starfish. / Mita, Masatoshi; Yamamoto, Kazutoshi; Yoshikuni, Michiyasu; Ohno, Kaoru; Nagahama, Yoshitaka.

In: General and Comparative Endocrinology, Vol. 153, No. 1-3, 01.01.2007, p. 299-301.

Research output: Contribution to journalArticle

Mita, Masatoshi ; Yamamoto, Kazutoshi ; Yoshikuni, Michiyasu ; Ohno, Kaoru ; Nagahama, Yoshitaka. / Preliminary study on the receptor of gonad-stimulating substance (GSS) as a gonadotropin of starfish. In: General and Comparative Endocrinology. 2007 ; Vol. 153, No. 1-3. pp. 299-301.
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