TY - JOUR
T1 - Preliminary study on the receptor of gonad-stimulating substance (GSS) as a gonadotropin of starfish
AU - Mita, Masatoshi
AU - Yamamoto, Kazutoshi
AU - Yoshikuni, Michiyasu
AU - Ohno, Kaoru
AU - Nagahama, Yoshitaka
N1 - Funding Information:
This study was carried out under the auspices of the National Institute for Basic Biology Cooperative Research Program (5-106 and 6-106 to M.M.) and supported in part by Grants-in-Aid for Scientific Research (C) (No. 18570056 to M.M. and No.16570059 to M.Y.) from the Japan Society for the Promotion of Science and for Scientific Research (B) (No. 16370032 to Y.N.) from MEXT, and by the academic research supporting program from Daiko Foundation (No. 9092 to M.Y.).
PY - 2007
Y1 - 2007
N2 - Previously, the gonad-stimulating substance (GSS) which acts as the gonadotropin was purified from the starfish (Asterina pectinifera) and subsequently, its structure was deciphered. In this study, artificial GSS was synthesized and its interaction with the receptors was examined further. According to competitive experiments using radioiodinated and radioinert GSS in various tissues of A. pectinifera, high specific bindings were observed in the ovarian follicle cells and testicular interstitial cells. Scatchard plot analysis also showed that Kd values were about 4 nM in follicle cells and about 7 nM in interstitial cells. The numbers of binding sites in follicle cells were estimated to be about 3 pmoles/mg protein and in interstitial cells to be about 1 pmoles/mg protein. These strongly suggest that GSS receptors are distributed to follicle cells in female and interstitial cells in male, respectively.
AB - Previously, the gonad-stimulating substance (GSS) which acts as the gonadotropin was purified from the starfish (Asterina pectinifera) and subsequently, its structure was deciphered. In this study, artificial GSS was synthesized and its interaction with the receptors was examined further. According to competitive experiments using radioiodinated and radioinert GSS in various tissues of A. pectinifera, high specific bindings were observed in the ovarian follicle cells and testicular interstitial cells. Scatchard plot analysis also showed that Kd values were about 4 nM in follicle cells and about 7 nM in interstitial cells. The numbers of binding sites in follicle cells were estimated to be about 3 pmoles/mg protein and in interstitial cells to be about 1 pmoles/mg protein. These strongly suggest that GSS receptors are distributed to follicle cells in female and interstitial cells in male, respectively.
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U2 - 10.1016/j.ygcen.2007.01.038
DO - 10.1016/j.ygcen.2007.01.038
M3 - Article
C2 - 17368456
AN - SCOPUS:34547134227
SN - 0016-6480
VL - 153
SP - 299
EP - 301
JO - General and Comparative Endocrinology
JF - General and Comparative Endocrinology
IS - 1-3
ER -