Preliminary time-course study of antiinflammatory macrophage infiltration in crush-injured skeletal muscle

Jun ichi Shono, Shohei Sakaguchi, Takahiro Suzuki, Mai Khoi Q. Do, Wataru Mizunoya, Mako Nakamura, Yusuke Sato, Mitsuhiro Furuse, Koji Yamada, Yoshihide Ikeuchi, Ryuichi Tatsumi

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Muscle damage induces massive macrophage infiltration of the injury site, in which activated pro-inflammatory and anti-inflammatory phenotypes (currently classified as M1 and M2, respectively) have been documented as distinct functional populations predominant at different times after the conventional acute injury by intramuscular injection of snake venoms (cardiotoxin, notexin) or chemicals (bupivacaine hydrochloride, barium chloride). The present study employed a muscle-crush injury model that may better reflect the physiologic damage and repair processes initiated by contusing a gastrocnemius muscle in the lower hind-limb of adult mice with hemostat forceps, and examined the time-course invasion of M1 and M2 macrophages during muscle regeneration by immunocytochemistry of CD197 and CD206 marker proteins. CD197-positive M1 macrophages were observed exclusively at 1-4 days after crush followed by the alternative prevalence of CD206-positive M2 at 7 days of myogenic differentiation, characterized by increasing levels of myogenin messenger RNA expression. Preliminary PCR analysis showed that M2 may produce hepatocyte growth factor (HGF) in culture, providing additional benefit to understanding that M2 populations actively promote regenerative myogenesis (muscle fiber repair) and moto-neuritogenesis (re-attachment of motoneuron terminals onto damaged fibers) through their time-specific infiltration and release of growth factor at the injury site early in muscle regeneration.

Original languageEnglish
Pages (from-to)744-750
Number of pages7
JournalAnimal Science Journal
Volume84
Issue number11
DOIs
Publication statusPublished - Nov 1 2013

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muscle development
skeletal muscle
Skeletal Muscle
macrophages
Anti-Inflammatory Agents
Macrophages
Muscles
muscles
hepatocyte growth factor
barium
venoms
immunocytochemistry
motor neurons
messenger RNA
Regeneration
intramuscular injection
limbs (animal)
Wounds and Injuries
muscle fibers
snakes

All Science Journal Classification (ASJC) codes

  • Agricultural and Biological Sciences(all)

Cite this

Preliminary time-course study of antiinflammatory macrophage infiltration in crush-injured skeletal muscle. / Shono, Jun ichi; Sakaguchi, Shohei; Suzuki, Takahiro; Do, Mai Khoi Q.; Mizunoya, Wataru; Nakamura, Mako; Sato, Yusuke; Furuse, Mitsuhiro; Yamada, Koji; Ikeuchi, Yoshihide; Tatsumi, Ryuichi.

In: Animal Science Journal, Vol. 84, No. 11, 01.11.2013, p. 744-750.

Research output: Contribution to journalArticle

Shono, Jun ichi ; Sakaguchi, Shohei ; Suzuki, Takahiro ; Do, Mai Khoi Q. ; Mizunoya, Wataru ; Nakamura, Mako ; Sato, Yusuke ; Furuse, Mitsuhiro ; Yamada, Koji ; Ikeuchi, Yoshihide ; Tatsumi, Ryuichi. / Preliminary time-course study of antiinflammatory macrophage infiltration in crush-injured skeletal muscle. In: Animal Science Journal. 2013 ; Vol. 84, No. 11. pp. 744-750.
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