Preparation and catalytic performance of surfactant-manganese peroxidase-MnII ternary complex in organic media

Shin Ya Okazaki, Masahiro Goto, Shintaro Furusaki, Hiroyuki Wariishi, Hiroo Tanaka

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

A novel preparation method for surfactant-MnP-MnII ternary complex utilizing water-in-oil emulsions has been developed. The surfactant-MnP complex was spectroscopically characterized, strongly suggesting that the heme environment of the surfactant-MnP complex in benzene is identical to that of native MnP in the aqueous buffer. o-Phenylenediamine oxidation catalyzed by the surfactant-MnP-MnII ternary complex was performed in benzene. The ternary complex efficiently catalyzed the oxidation, and the complex was catalytically stable. Kinetic experiments revealed that the reaction mechanism was as follows: MnP is oxidized by H2O2 and the oxidized intermediate catalyzes the oxidation of MnII to MnIII and the latter, after complexed with malonate, readily oxidizes o-PDA inside the complex. Thus, the organic substrate o-PDA, but not MnIII, shuttled between the surfactant-MnP-MnII ternary complex and organic solvent.

Original languageEnglish
Pages (from-to)329-332
Number of pages4
JournalEnzyme and Microbial Technology
Volume28
Issue number4-5
DOIs
Publication statusPublished - Mar 8 2001

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology

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