TY - JOUR
T1 - Preparation and catalytic performance of surfactant-manganese peroxidase-MnII ternary complex in organic media
AU - Okazaki, Shin Ya
AU - Goto, Masahiro
AU - Furusaki, Shintaro
AU - Wariishi, Hiroyuki
AU - Tanaka, Hiroo
N1 - Funding Information:
This research was supported by a Grant-in-Aid for Scientific Research (B) (10555284) from the Ministry of Education, Science, Sports and Culture of Japan (to M.G. and S.F.), Kyushu University Interdisciplinary Programs in Education and Projects in Research Development (to H.W. and M.G.) and the Proposal-Based New Industry Creative Type Technology R&D Promotion Program from the New Energy and Industrial Technology Development Organization (NEDO) of Japan (to S.F., M.G., H.W. and H.T.).
Funding Information:
S.O. was supported by Research Fellowships of the Japan Society for the Promotion of Science for Young Scientists.
PY - 2001/3/8
Y1 - 2001/3/8
N2 - A novel preparation method for surfactant-MnP-MnII ternary complex utilizing water-in-oil emulsions has been developed. The surfactant-MnP complex was spectroscopically characterized, strongly suggesting that the heme environment of the surfactant-MnP complex in benzene is identical to that of native MnP in the aqueous buffer. o-Phenylenediamine oxidation catalyzed by the surfactant-MnP-MnII ternary complex was performed in benzene. The ternary complex efficiently catalyzed the oxidation, and the complex was catalytically stable. Kinetic experiments revealed that the reaction mechanism was as follows: MnP is oxidized by H2O2 and the oxidized intermediate catalyzes the oxidation of MnII to MnIII and the latter, after complexed with malonate, readily oxidizes o-PDA inside the complex. Thus, the organic substrate o-PDA, but not MnIII, shuttled between the surfactant-MnP-MnII ternary complex and organic solvent.
AB - A novel preparation method for surfactant-MnP-MnII ternary complex utilizing water-in-oil emulsions has been developed. The surfactant-MnP complex was spectroscopically characterized, strongly suggesting that the heme environment of the surfactant-MnP complex in benzene is identical to that of native MnP in the aqueous buffer. o-Phenylenediamine oxidation catalyzed by the surfactant-MnP-MnII ternary complex was performed in benzene. The ternary complex efficiently catalyzed the oxidation, and the complex was catalytically stable. Kinetic experiments revealed that the reaction mechanism was as follows: MnP is oxidized by H2O2 and the oxidized intermediate catalyzes the oxidation of MnII to MnIII and the latter, after complexed with malonate, readily oxidizes o-PDA inside the complex. Thus, the organic substrate o-PDA, but not MnIII, shuttled between the surfactant-MnP-MnII ternary complex and organic solvent.
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U2 - 10.1016/S0141-0229(00)00344-6
DO - 10.1016/S0141-0229(00)00344-6
M3 - Article
AN - SCOPUS:0035826278
SN - 0141-0229
VL - 28
SP - 329
EP - 332
JO - Enzyme and Microbial Technology
JF - Enzyme and Microbial Technology
IS - 4-5
ER -