Preparation and characterization of a VEGF-Fc fusion protein matrix for enhancing HUVEC growth

Meihua Yu, Fengyi Du, Hirohiko Ise, Wei Zhao, Yan Zhang, Yaoting Yu, Fanglian Yao, Jun Yang, Toshihiro Akaike

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

To enhance vascularization of hydrophobic implants in vivo, a VEGF-Fc fusion protein consisting of vascular endothelial growth factor (VEGF) fused to the immunoglobulin G Fc domain was prepared as an artificial extracellular matrix (ECM). VEGF-Fc was stably immobilized on a polystyrene plate due to the hydrophobicity of the Fc domain, and significantly enhanced the adhesion of human umbilical vein endothelial cells (HUVECs). Additionally, the use of VEGF-Fc as an ECM markedly promoted the proliferation of HUVECs longer than 72 h and induced the reorganization of actin filaments into larger stress fibers within these cells. The VEGF-Fc fusion protein may be a promising artificial ECM for enhancing endothelial cell growth.

Original languageEnglish
Pages (from-to)1765-1771
Number of pages7
JournalBiotechnology letters
Volume34
Issue number9
DOIs
Publication statusPublished - Oct 1 2012
Externally publishedYes

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Endothelial cells
Human Umbilical Vein Endothelial Cells
Cell growth
Vascular Endothelial Growth Factor A
Fusion reactions
Proteins
Extracellular Matrix
Growth
Stress Fibers
Polystyrenes
Hydrophobicity
Actin Cytoskeleton
Hydrophobic and Hydrophilic Interactions
Actins
Adhesion
Endothelial Cells
Immunoglobulin G
Intercellular Signaling Peptides and Proteins
Fibers

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Cite this

Preparation and characterization of a VEGF-Fc fusion protein matrix for enhancing HUVEC growth. / Yu, Meihua; Du, Fengyi; Ise, Hirohiko; Zhao, Wei; Zhang, Yan; Yu, Yaoting; Yao, Fanglian; Yang, Jun; Akaike, Toshihiro.

In: Biotechnology letters, Vol. 34, No. 9, 01.10.2012, p. 1765-1771.

Research output: Contribution to journalArticle

Yu, M, Du, F, Ise, H, Zhao, W, Zhang, Y, Yu, Y, Yao, F, Yang, J & Akaike, T 2012, 'Preparation and characterization of a VEGF-Fc fusion protein matrix for enhancing HUVEC growth', Biotechnology letters, vol. 34, no. 9, pp. 1765-1771. https://doi.org/10.1007/s10529-012-0959-7
Yu, Meihua ; Du, Fengyi ; Ise, Hirohiko ; Zhao, Wei ; Zhang, Yan ; Yu, Yaoting ; Yao, Fanglian ; Yang, Jun ; Akaike, Toshihiro. / Preparation and characterization of a VEGF-Fc fusion protein matrix for enhancing HUVEC growth. In: Biotechnology letters. 2012 ; Vol. 34, No. 9. pp. 1765-1771.
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