Preparation and characterization of porous alginate scaffolds containing various amounts of octacalcium phosphate (OCP) crystals

Naru Shiraishi, Takahisa Anada, Yoshitomo Honda, Taisuke Masuda, Keiichi Sasaki, Osamu Suzuki

Research output: Contribution to journalArticlepeer-review

17 Citations (Scopus)

Abstract

The present study was designed to investigate whether the amount of octacalcium phosphate (OCP) affects the characteristics of alginate (Alg)/OCP scaffolds regarding the pore formation and its distribution, and the thermodynamic stability from OCP to hydroxyapatite (HA) in an in vitro physiological environment. Alg/OCP composites with weight ratios of 100/0, 75/25, 50/50, and 25/75 were prepared through mixing the ground synthesized OCP crystals with an Alg solution and applying lyophilization. Analysis of X-ray diffraction, Fourier transform infrared (FTIR) spectroscopy, and mercury intrusion porosimetry verified that the crystalline phase of OCP and the porosities were retained regardless of the OCP amount. On the other hand, the elastic modulus, determined by mechanical testing, and, interestingly, the pore size increased with increasing the OCP amount. The immersion of the composites in a simulated body fluid up to 14 days revealed that OCP in Alg matrices tends to convert to HA with enhancing the calcium consumption depending on the OCP amount. The results indicated that the inclusion of OCP crystals in the Alg matrix by the mixing process controls the character of the pore distribution in Alg/OCP composites while maintaining the transitory nature of OCP.

Original languageEnglish
Pages (from-to)907-914
Number of pages8
JournalJournal of Materials Science: Materials in Medicine
Volume21
Issue number3
DOIs
Publication statusPublished - Mar 1 2010
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Bioengineering
  • Biomaterials
  • Biomedical Engineering

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