Preparation and characterization of thermo-responsive albumin nanospheres

Zhe Yu Shen, Guang Hui Ma, Toshiaki Dobashi, Yasuyuki Maki, Zhi Guo Su

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

Thermo-responsive poly(N-isopropylacrylamide-co-acrylamide)-block-polyallylamine-conjugated albumin nanospheres (PAN), new thermal targeting anti-cancer drug carrier, was developed by conjugating poly(N-isopropylacrylamide-co-acrylamide)-block-polyallylamine (PNIPAM-AAm-b-PAA) on the surface of albumin nanospheres (AN). PAN may selectively accumulate onto solid tumors that are maintained above physiological temperature due to local hyperthermia. PNIPAM-AAm-b-PAA was synthesized by radical polymerization, and AN was prepared by ultrasonic emulsification. AN with diameter below 200 nm and narrow size distribution was obtained by optimizing the preparative conditions. Rose Bengal (RB) was used as model drug for entrapment into the AN and PAN during the particle preparation. The release rate of RB from PAN compared with AN in trypsin solution was slower, and decreased with the increase of PNIPAM-AAm-b-PAA molecular weight, which suggested that the existence of a steric hydrophilic barrier on AN made digestion of AN more difficult. Moreover, the release of RB from PAN above the cloud-point temperature (Tcp) of PNIPAM-AAm-b-PAA became faster. This was because the density of temperature-responsive polymers on AN was not so high, so that the interspace between the polymer chains increased after they shrunk due to the high temperature. As a result, the biodegradable AN was attacked more easily by trypsin. The design of PAN overcame the disadvantages of temperature-responsive polymeric micelles.

Original languageEnglish
Pages (from-to)133-142
Number of pages10
JournalInternational Journal of Pharmaceutics
Volume346
Issue number1-2
DOIs
Publication statusPublished - Jan 4 2008
Externally publishedYes

Fingerprint

Nanospheres
Albumins
Rose Bengal
Temperature
Trypsin
Polymers
Induced Hyperthermia
Drug Carriers
Micelles
Ultrasonics
Polymerization
Digestion
Neoplasms
Hot Temperature
Molecular Weight

All Science Journal Classification (ASJC) codes

  • Pharmaceutical Science

Cite this

Preparation and characterization of thermo-responsive albumin nanospheres. / Shen, Zhe Yu; Ma, Guang Hui; Dobashi, Toshiaki; Maki, Yasuyuki; Su, Zhi Guo.

In: International Journal of Pharmaceutics, Vol. 346, No. 1-2, 04.01.2008, p. 133-142.

Research output: Contribution to journalArticle

Shen, Zhe Yu ; Ma, Guang Hui ; Dobashi, Toshiaki ; Maki, Yasuyuki ; Su, Zhi Guo. / Preparation and characterization of thermo-responsive albumin nanospheres. In: International Journal of Pharmaceutics. 2008 ; Vol. 346, No. 1-2. pp. 133-142.
@article{d9b45fe411f54555984f8ce221f84499,
title = "Preparation and characterization of thermo-responsive albumin nanospheres",
abstract = "Thermo-responsive poly(N-isopropylacrylamide-co-acrylamide)-block-polyallylamine-conjugated albumin nanospheres (PAN), new thermal targeting anti-cancer drug carrier, was developed by conjugating poly(N-isopropylacrylamide-co-acrylamide)-block-polyallylamine (PNIPAM-AAm-b-PAA) on the surface of albumin nanospheres (AN). PAN may selectively accumulate onto solid tumors that are maintained above physiological temperature due to local hyperthermia. PNIPAM-AAm-b-PAA was synthesized by radical polymerization, and AN was prepared by ultrasonic emulsification. AN with diameter below 200 nm and narrow size distribution was obtained by optimizing the preparative conditions. Rose Bengal (RB) was used as model drug for entrapment into the AN and PAN during the particle preparation. The release rate of RB from PAN compared with AN in trypsin solution was slower, and decreased with the increase of PNIPAM-AAm-b-PAA molecular weight, which suggested that the existence of a steric hydrophilic barrier on AN made digestion of AN more difficult. Moreover, the release of RB from PAN above the cloud-point temperature (Tcp) of PNIPAM-AAm-b-PAA became faster. This was because the density of temperature-responsive polymers on AN was not so high, so that the interspace between the polymer chains increased after they shrunk due to the high temperature. As a result, the biodegradable AN was attacked more easily by trypsin. The design of PAN overcame the disadvantages of temperature-responsive polymeric micelles.",
author = "Shen, {Zhe Yu} and Ma, {Guang Hui} and Toshiaki Dobashi and Yasuyuki Maki and Su, {Zhi Guo}",
year = "2008",
month = "1",
day = "4",
doi = "10.1016/j.ijpharm.2007.06.004",
language = "English",
volume = "346",
pages = "133--142",
journal = "International Journal of Pharmaceutics",
issn = "0378-5173",
publisher = "Elsevier",
number = "1-2",

}

TY - JOUR

T1 - Preparation and characterization of thermo-responsive albumin nanospheres

AU - Shen, Zhe Yu

AU - Ma, Guang Hui

AU - Dobashi, Toshiaki

AU - Maki, Yasuyuki

AU - Su, Zhi Guo

PY - 2008/1/4

Y1 - 2008/1/4

N2 - Thermo-responsive poly(N-isopropylacrylamide-co-acrylamide)-block-polyallylamine-conjugated albumin nanospheres (PAN), new thermal targeting anti-cancer drug carrier, was developed by conjugating poly(N-isopropylacrylamide-co-acrylamide)-block-polyallylamine (PNIPAM-AAm-b-PAA) on the surface of albumin nanospheres (AN). PAN may selectively accumulate onto solid tumors that are maintained above physiological temperature due to local hyperthermia. PNIPAM-AAm-b-PAA was synthesized by radical polymerization, and AN was prepared by ultrasonic emulsification. AN with diameter below 200 nm and narrow size distribution was obtained by optimizing the preparative conditions. Rose Bengal (RB) was used as model drug for entrapment into the AN and PAN during the particle preparation. The release rate of RB from PAN compared with AN in trypsin solution was slower, and decreased with the increase of PNIPAM-AAm-b-PAA molecular weight, which suggested that the existence of a steric hydrophilic barrier on AN made digestion of AN more difficult. Moreover, the release of RB from PAN above the cloud-point temperature (Tcp) of PNIPAM-AAm-b-PAA became faster. This was because the density of temperature-responsive polymers on AN was not so high, so that the interspace between the polymer chains increased after they shrunk due to the high temperature. As a result, the biodegradable AN was attacked more easily by trypsin. The design of PAN overcame the disadvantages of temperature-responsive polymeric micelles.

AB - Thermo-responsive poly(N-isopropylacrylamide-co-acrylamide)-block-polyallylamine-conjugated albumin nanospheres (PAN), new thermal targeting anti-cancer drug carrier, was developed by conjugating poly(N-isopropylacrylamide-co-acrylamide)-block-polyallylamine (PNIPAM-AAm-b-PAA) on the surface of albumin nanospheres (AN). PAN may selectively accumulate onto solid tumors that are maintained above physiological temperature due to local hyperthermia. PNIPAM-AAm-b-PAA was synthesized by radical polymerization, and AN was prepared by ultrasonic emulsification. AN with diameter below 200 nm and narrow size distribution was obtained by optimizing the preparative conditions. Rose Bengal (RB) was used as model drug for entrapment into the AN and PAN during the particle preparation. The release rate of RB from PAN compared with AN in trypsin solution was slower, and decreased with the increase of PNIPAM-AAm-b-PAA molecular weight, which suggested that the existence of a steric hydrophilic barrier on AN made digestion of AN more difficult. Moreover, the release of RB from PAN above the cloud-point temperature (Tcp) of PNIPAM-AAm-b-PAA became faster. This was because the density of temperature-responsive polymers on AN was not so high, so that the interspace between the polymer chains increased after they shrunk due to the high temperature. As a result, the biodegradable AN was attacked more easily by trypsin. The design of PAN overcame the disadvantages of temperature-responsive polymeric micelles.

UR - http://www.scopus.com/inward/record.url?scp=36048944478&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=36048944478&partnerID=8YFLogxK

U2 - 10.1016/j.ijpharm.2007.06.004

DO - 10.1016/j.ijpharm.2007.06.004

M3 - Article

C2 - 17651929

AN - SCOPUS:36048944478

VL - 346

SP - 133

EP - 142

JO - International Journal of Pharmaceutics

JF - International Journal of Pharmaceutics

SN - 0378-5173

IS - 1-2

ER -