Preparation of β(1→6)-linked galactofuranoside oligomers from the acidic polysaccharide of Fusarium sp. M7-1

Nahrowi Ramli, Hironao Shinohara, Kaoru Takegawa, Shojiro Iwahara

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

This paper describes a method of preparating β(1→6)-linked galactofuranoside oligomers. The main structure of the acidic polysaccharide of Fusarium sp. M7-1 has a linear chain composed of β(1→6)-linked galactofuranose residues. For preparation of the β(1→6) galactofuranoside oligomers, glucuronic acid residues were converted to unsaturated sugars using an acidic polysaccharide lyase from Cellulomonas sp. Mild acid hydrolysis was found to be very effective in preparing the β(1→6) galactofuranoside oligomers from the unsaturated polysaccharide of Fusarium sp. M7-1. After treatment with 1 M acetic acid at 100°C for 5 h, the unsaturated polysaccharide was separated into oligomers, and the primary structures of the β(1→6) galactofuranoside oligomers were resolved, mainly by 400-MHz 1H-NMR spectrometry. These oligomers were resistant to commercial β-d-galactosidases.

Original languageEnglish
Pages (from-to)341-345
Number of pages5
JournalJournal of Fermentation and Bioengineering
Volume78
Issue number5
DOIs
Publication statusPublished - 1994
Externally publishedYes

Fingerprint

Fusarium
Polysaccharides
Oligomers
Polysaccharide-Lyases
Cellulomonas
Galactosidases
Glucuronic Acid
Acetic Acid
Spectrum Analysis
Hydrolysis
Acids
Acetic acid
Sugars
Spectrometry
Nuclear magnetic resonance

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

Preparation of β(1→6)-linked galactofuranoside oligomers from the acidic polysaccharide of Fusarium sp. M7-1. / Ramli, Nahrowi; Shinohara, Hironao; Takegawa, Kaoru; Iwahara, Shojiro.

In: Journal of Fermentation and Bioengineering, Vol. 78, No. 5, 1994, p. 341-345.

Research output: Contribution to journalArticle

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