Preparation of a Human Serum Based Control Material Specific for Lipid Assay

Yuzo Kayamori, Minoru Tedokon, Yoshiaki Katayama

Research output: Contribution to journalArticle

Abstract

We herein describe a procedure for manufacturing the human serum based control specific for lipid assay, applying the precipitation method by Burstein et al. The control serum is prepared by reconstituting a lyophilized LDL and VLDL depleted serum with distilled water and adding a liquefied LDL and VLDL components to it when in use. The advantageous point this procedure is that the concentration of each lipid component can be changed according to the requirement. The control serum can equally be compared with fresh human serum in its transparency, electrophoretic characteristics and recovery of lipids by ultracentrifugal analysis. Furthermore, the enzymatic analyses of triglycerides, total cholesterol and phospholipids in the control serum the similar reaction kinetics as those of fresh human serum.

Original languageEnglish
Pages (from-to)176-182
Number of pages7
JournalJapanese Journal of Clinical Chemistry
Volume19
Issue number2
DOIs
Publication statusPublished - Jan 1 1990
Externally publishedYes

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Assays
Lipids
Serum
Reaction kinetics
Transparency
Phospholipids
Triglycerides
Cholesterol
Recovery
Water
oxidized low density lipoprotein

All Science Journal Classification (ASJC) codes

  • Clinical Biochemistry

Cite this

Preparation of a Human Serum Based Control Material Specific for Lipid Assay. / Kayamori, Yuzo; Tedokon, Minoru; Katayama, Yoshiaki.

In: Japanese Journal of Clinical Chemistry, Vol. 19, No. 2, 01.01.1990, p. 176-182.

Research output: Contribution to journalArticle

Kayamori, Yuzo ; Tedokon, Minoru ; Katayama, Yoshiaki. / Preparation of a Human Serum Based Control Material Specific for Lipid Assay. In: Japanese Journal of Clinical Chemistry. 1990 ; Vol. 19, No. 2. pp. 176-182.
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