Preparation of a murine cell line which stably expresses human T lymphotropic virus type I (HTLV-I) env genome products

Tatsuroh Joh, Masatoshi Fujita, Yuetsu Tanaka, Hiroshi Shiku

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1 Citation (Scopus)

Abstract

We prepared the murine myeloma cell line NS-1, which stably expressed the human T lymphotropic virus type I (HTLV-I) env gene. The plasmid BCMGEnv was constructed from the episomal vector BCMGSNeo, which was primarily derived from bovine papilloma virus. Transfected env expression was detected by Northern blotting, as well as by flow cytometry using envelope protein-specific monoclonal antibodies (mAb). Expression was detectable for at least seven months. The env transfectants induced syncytium formation which is characteristic of HTLV-I-infected cells, in the human uterine cervical cancer line, HeLa, and the rat cell line, XC. The requirement of envelope proteins for syncytium formation was confirmed by an inhibition assay with envelope protein-specific mAb. Therefore, env transfectants are not only stable, but also have its specific biological function. This system may be useful to analyze the initial steps of viral attachment to the cell surface and to search for the HTLV-I receptor.

Original languageEnglish
Pages (from-to)227-230
Number of pages4
JournalGene
Volume161
Issue number2
DOIs
Publication statusPublished - Aug 19 1995
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Genetics

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