Preparation of a naturally occurring D-erythro-(2S,3R)- sphingosylphosphocholine using Shewanella alga NS-589

Noriyuki Sueyoshi, Hiroyuki Izu, Makoto Ito

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Sphingosylphosphocholine, an N-deacylated derivative of sphingomyelin, has been found to be involved in many cellular events. This paper describes a new method for preparation of a D-erythro-sphingosylphosphocholine, which is naturally occurring but difficult to prepare by chemical methods, using marine bacteria as a biocatalyst. When cultured with Shewanella alga NS-589 in synthetic medium, sphingomyelin was found to be efficiently converted by sphingomyelin deacylase to sphingosylphosphocholine. Sphingosylphosphocholine was purified with a high yield from the culture supernatant and identified to be a D-erythro-(2S,3R)-isomer containing d18:1 sphingenine as a long-chain base by fast atom bombardment mass spectrometry and NMR analyses.

Original languageEnglish
Pages (from-to)1923-1927
Number of pages5
JournalJournal of Lipid Research
Volume38
Issue number9
Publication statusPublished - Sep 1 1997

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Endocrinology
  • Cell Biology

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