Preparation of a novel monoclonal antibody against the antimalarial drugs, artemisinin and artesunate

Hiroyuki Tanaka, Waraporn Putalun, Wanchai De-Eknamkul, Oraphan Matangkasombut, Yukihiro Shoyama

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13 Citations (Scopus)

Abstract

A monoclonal antibody (MAb) 1C1 against artemisinin was prepared by a cell fusion with splenocytes and aminopterin-sensitive myeloma cells, SP2/0. An artesunate-BSA conjugate was used as immunogen to raise antibodies specific to artemisinin. The prepared anti-artemisinin MAb-1C1 have a novel characteristic which shows a specificity to compounds that are structurally related to artemisinin such as artesunate (630%) and dihydroartemisinin (29.9%). By using MAb-1C1, a specific and reliable ELISA was developed for the detection of compounds structurally related to artemisinin. The system shows a full measuring range from 2 to 20 μg/mL in the case of artemisinin and from 4 to 125 ng/mL in the case of artesunate in the competitive ELISA, and was validated to be of use for surveying and breeding of Artemisia annua containing a high amount of artemisinin as well as for the characterization of the pharmacokinetics of artemisinin and its derivatives.

Original languageEnglish
Pages (from-to)1127-1132
Number of pages6
JournalPlanta Medica
Volume73
Issue number10
DOIs
Publication statusPublished - Aug 1 2007

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Molecular Medicine
  • Pharmacology
  • Pharmaceutical Science
  • Drug Discovery
  • Complementary and alternative medicine
  • Organic Chemistry

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    Tanaka, H., Putalun, W., De-Eknamkul, W., Matangkasombut, O., & Shoyama, Y. (2007). Preparation of a novel monoclonal antibody against the antimalarial drugs, artemisinin and artesunate. Planta Medica, 73(10), 1127-1132. https://doi.org/10.1055/s-2007-981560