Preparation of a novel monoclonal antibody against the antimalarial drugs, artemisinin and artesunate

Hiroyuki Tanaka; Waraporn Putalun; Wanchai De-Eknamkul; Oraphan Matangkasombut; Yukihiro Shoyama

doi:10.1055/s-2007-981560

Preparation of a novel monoclonal antibody against the antimalarial drugs, artemisinin and artesunate

Hiroyuki Tanaka, Waraporn Putalun, Wanchai De-Eknamkul, Oraphan Matangkasombut, Yukihiro Shoyama

Pharmaceutical Health Care and Sciences

Research output: Contribution to journal › Article

13 Citations (Scopus)

Abstract

A monoclonal antibody (MAb) 1C1 against artemisinin was prepared by a cell fusion with splenocytes and aminopterin-sensitive myeloma cells, SP2/0. An artesunate-BSA conjugate was used as immunogen to raise antibodies specific to artemisinin. The prepared anti-artemisinin MAb-1C1 have a novel characteristic which shows a specificity to compounds that are structurally related to artemisinin such as artesunate (630%) and dihydroartemisinin (29.9%). By using MAb-1C1, a specific and reliable ELISA was developed for the detection of compounds structurally related to artemisinin. The system shows a full measuring range from 2 to 20 μg/mL in the case of artemisinin and from 4 to 125 ng/mL in the case of artesunate in the competitive ELISA, and was validated to be of use for surveying and breeding of Artemisia annua containing a high amount of artemisinin as well as for the characterization of the pharmacokinetics of artemisinin and its derivatives.

Original language	English
Pages (from-to)	1127-1132
Number of pages	6
Journal	Planta Medica
Volume	73
Issue number	10
DOIs	https://doi.org/10.1055/s-2007-981560
Publication status	Published - Aug 1 2007

All Science Journal Classification (ASJC) codes

Analytical Chemistry
Molecular Medicine
Pharmacology
Pharmaceutical Science
Drug Discovery
Complementary and alternative medicine
Organic Chemistry

Access to Document

10.1055/s-2007-981560

Fingerprint Dive into the research topics of 'Preparation of a novel monoclonal antibody against the antimalarial drugs, artemisinin and artesunate'. Together they form a unique fingerprint.

Cite this

Tanaka, H., Putalun, W., De-Eknamkul, W., Matangkasombut, O., & Shoyama, Y. (2007). Preparation of a novel monoclonal antibody against the antimalarial drugs, artemisinin and artesunate. Planta Medica, 73(10), 1127-1132. https://doi.org/10.1055/s-2007-981560

@article{fa30abc1899447128be9fdbaa3248a03,

title = "Preparation of a novel monoclonal antibody against the antimalarial drugs, artemisinin and artesunate",

abstract = "A monoclonal antibody (MAb) 1C1 against artemisinin was prepared by a cell fusion with splenocytes and aminopterin-sensitive myeloma cells, SP2/0. An artesunate-BSA conjugate was used as immunogen to raise antibodies specific to artemisinin. The prepared anti-artemisinin MAb-1C1 have a novel characteristic which shows a specificity to compounds that are structurally related to artemisinin such as artesunate (630%) and dihydroartemisinin (29.9%). By using MAb-1C1, a specific and reliable ELISA was developed for the detection of compounds structurally related to artemisinin. The system shows a full measuring range from 2 to 20 μg/mL in the case of artemisinin and from 4 to 125 ng/mL in the case of artesunate in the competitive ELISA, and was validated to be of use for surveying and breeding of Artemisia annua containing a high amount of artemisinin as well as for the characterization of the pharmacokinetics of artemisinin and its derivatives.",

author = "Hiroyuki Tanaka and Waraporn Putalun and Wanchai De-Eknamkul and Oraphan Matangkasombut and Yukihiro Shoyama",

year = "2007",

month = aug,

day = "1",

doi = "10.1055/s-2007-981560",

language = "English",

volume = "73",

pages = "1127--1132",

journal = "Planta Medica",

issn = "0032-0943",

publisher = "Georg Thieme Verlag",

number = "10",

}

TY - JOUR

T1 - Preparation of a novel monoclonal antibody against the antimalarial drugs, artemisinin and artesunate

AU - Tanaka, Hiroyuki

AU - Putalun, Waraporn

AU - De-Eknamkul, Wanchai

AU - Matangkasombut, Oraphan

AU - Shoyama, Yukihiro

PY - 2007/8/1

Y1 - 2007/8/1

N2 - A monoclonal antibody (MAb) 1C1 against artemisinin was prepared by a cell fusion with splenocytes and aminopterin-sensitive myeloma cells, SP2/0. An artesunate-BSA conjugate was used as immunogen to raise antibodies specific to artemisinin. The prepared anti-artemisinin MAb-1C1 have a novel characteristic which shows a specificity to compounds that are structurally related to artemisinin such as artesunate (630%) and dihydroartemisinin (29.9%). By using MAb-1C1, a specific and reliable ELISA was developed for the detection of compounds structurally related to artemisinin. The system shows a full measuring range from 2 to 20 μg/mL in the case of artemisinin and from 4 to 125 ng/mL in the case of artesunate in the competitive ELISA, and was validated to be of use for surveying and breeding of Artemisia annua containing a high amount of artemisinin as well as for the characterization of the pharmacokinetics of artemisinin and its derivatives.

AB - A monoclonal antibody (MAb) 1C1 against artemisinin was prepared by a cell fusion with splenocytes and aminopterin-sensitive myeloma cells, SP2/0. An artesunate-BSA conjugate was used as immunogen to raise antibodies specific to artemisinin. The prepared anti-artemisinin MAb-1C1 have a novel characteristic which shows a specificity to compounds that are structurally related to artemisinin such as artesunate (630%) and dihydroartemisinin (29.9%). By using MAb-1C1, a specific and reliable ELISA was developed for the detection of compounds structurally related to artemisinin. The system shows a full measuring range from 2 to 20 μg/mL in the case of artemisinin and from 4 to 125 ng/mL in the case of artesunate in the competitive ELISA, and was validated to be of use for surveying and breeding of Artemisia annua containing a high amount of artemisinin as well as for the characterization of the pharmacokinetics of artemisinin and its derivatives.

UR - http://www.scopus.com/inward/record.url?scp=34548652126&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34548652126&partnerID=8YFLogxK

U2 - 10.1055/s-2007-981560

DO - 10.1055/s-2007-981560

M3 - Article

C2 - 17611929

AN - SCOPUS:34548652126

VL - 73

SP - 1127

EP - 1132

JO - Planta Medica

JF - Planta Medica

SN - 0032-0943

IS - 10

ER -