Preparation of antiserum against rat Δ6-desaturase and its use to evaluate the desaturase protein levels in rats treated with gemfibrozil, a ligand for peroxisome proliferator-…

Yoko Shoji, Ayako Sanekata, Masao Sato, Katsumi Imaizumi

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Anti-rat Δ6-desaturase serum was produced by immunizing rabbits with the 14 N-terminal amino acids (2-15) of rat Δ6-desaturase. The antiserum prevented the enzymatic activity of Δ6-desaturase in microsomes. Subsequently, the antiserum was used to demonstrate that gemfibrozil, a ligand for peroxisome proliferator-activated receptor α, is involved in activating Δ6-desaturase gene expression, thereby elevating the protein level and the activity.

Original languageEnglish
Pages (from-to)1177-1178
Number of pages2
JournalBioscience, Biotechnology and Biochemistry
Volume67
Issue number5
DOIs
Publication statusPublished - Jan 1 2003

Fingerprint

Gemfibrozil
Peroxisome Proliferators
Rats
Immune Sera
Ligands
Peroxisome Proliferator-Activated Receptors
Microsomes
Gene expression
Proteins
Rabbits
Gene Expression
Amino Acids
Serum

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

Cite this

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