Preparation of artificial metalloenzymes by insertion of chromium(III) Schiff base complexes into apomyoglobin mutants

Masataka Ohashi, Tomomi Koshiyama, Takafumi Ueno, Manabu Yanase, Hiroshi Fujii, Yoshihito Watanabe

Research output: Contribution to journalArticle

190 Citations (Scopus)

Abstract

Insertion of a symmetric metal complex, [CrIII(5,5′-tBu-salophen)]+ (H2salophen = N,N′-bis(salicylidene)-1,2-phenylenediamine), into the active site of apomyoglobin is demonstrated (see picture). The metal ion and the ligand structure are very important factors that influence the binding affinity of the metal complex with the myoglobin (Mb) cavity. Semisynthetic metalloenzymes can catalyze enantioselective sulfoxidation by using the chiral protein cavity.

Original languageEnglish
Pages (from-to)1005-1008
Number of pages4
JournalAngewandte Chemie - International Edition
Volume42
Issue number9
DOIs
Publication statusPublished - Mar 3 2003

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Chemistry(all)

Fingerprint Dive into the research topics of 'Preparation of artificial metalloenzymes by insertion of chromium(III) Schiff base complexes into apomyoglobin mutants'. Together they form a unique fingerprint.

  • Cite this