Preparation of Biologically Active and Site Specifically Radioiodinated Recombinant Human Insulin-like Growth Factor-I.

Yasuhiro Kohama, Masaru Okabe, Kazutake Tsujikawa, Hiroaki Oka, Tetsuyuki Teramoto, Yuzo Kayamori, Masaaki Itoh, Tsutomu Mimura

Research output: Contribution to journalArticle

Abstract

Recombinant human insulin-like growth factor-I (rhIGF-I) was iodinated using a lactoperoxidase-catalyzed labeling method. The labeled products were separated into more than five fractions by ion-paired reverse-phase high performance liquid chromatography (HPLC). A fraction (peak 1), which showed the highest yield and radiactivity, was found to be biologically active in the BALB/c 3Ts cell proliferating system. The site of the iodination was investigated by S-pyridylethylation followed by trypsinization and separation with HPLC using reverse phase columns. From the amino acid analysis of the peaks which were radioactive, the iodination site of peak 1 was revealed to be Try-24 and Try-60. This is the first report of the biological activity of radioactive peptide hormone with a defined laveled site.
Original languageEnglish
Pages (from-to)808-810
Number of pages3
JournalChemical and Pharmaceutical Bulletin
Volume40
Issue number3
DOIs
Publication statusPublished - 1992

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