Preparation of Biologically Active and Site Specifically Radioiodinated Recombinant Human Insulin-like Growth Factor-I.

Yasuhiro Kohama, Masaru Okabe, Kazutake Tsujikawa, Hiroaki Oka, Tetsuyuki Teramoto, Yuzo Kayamori, Masaaki Itoh, Tsutomu Mimura

Research output: Contribution to journalArticle

Abstract

Recombinant human insulin-like growth factor-I (rhIGF-I) was iodinated using a lactoperoxidase-catalyzed labeling method. The labeled products were separated into more than five fractions by ion-paired reverse-phase high performance liquid chromatography (HPLC). A fraction (peak 1), which showed the highest yield and radiactivity, was found to be biologically active in the BALB/c 3Ts cell proliferating system. The site of the iodination was investigated by S-pyridylethylation followed by trypsinization and separation with HPLC using reverse phase columns. From the amino acid analysis of the peaks which were radioactive, the iodination site of peak 1 was revealed to be Try-24 and Try-60. This is the first report of the biological activity of radioactive peptide hormone with a defined laveled site.
Original languageEnglish
Pages (from-to)808-810
Number of pages3
JournalChemical and Pharmaceutical Bulletin
Volume40
Issue number3
DOIs
Publication statusPublished - 1992

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Halogenation
High performance liquid chromatography
Insulin-Like Growth Factor I
Catalytic Domain
High Pressure Liquid Chromatography
Lactoperoxidase
Peptide Hormones
Reverse-Phase Chromatography
Bioactivity
Labeling
Ions
Amino Acids

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Preparation of Biologically Active and Site Specifically Radioiodinated Recombinant Human Insulin-like Growth Factor-I. / Kohama, Yasuhiro; Okabe, Masaru; Tsujikawa, Kazutake; Oka, Hiroaki; Teramoto, Tetsuyuki; Kayamori, Yuzo; Itoh, Masaaki; Mimura, Tsutomu.

In: Chemical and Pharmaceutical Bulletin, Vol. 40, No. 3, 1992, p. 808-810.

Research output: Contribution to journalArticle

Kohama, Y, Okabe, M, Tsujikawa, K, Oka, H, Teramoto, T, Kayamori, Y, Itoh, M & Mimura, T 1992, 'Preparation of Biologically Active and Site Specifically Radioiodinated Recombinant Human Insulin-like Growth Factor-I.', Chemical and Pharmaceutical Bulletin, vol. 40, no. 3, pp. 808-810. https://doi.org/10.1248/cpb.40.808
Kohama, Yasuhiro ; Okabe, Masaru ; Tsujikawa, Kazutake ; Oka, Hiroaki ; Teramoto, Tetsuyuki ; Kayamori, Yuzo ; Itoh, Masaaki ; Mimura, Tsutomu. / Preparation of Biologically Active and Site Specifically Radioiodinated Recombinant Human Insulin-like Growth Factor-I. In: Chemical and Pharmaceutical Bulletin. 1992 ; Vol. 40, No. 3. pp. 808-810.
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